Negative regulation of fibroblast motility by Ena/VASP proteins

被引：381

作者：

Bear, JE

Loureiro, JJ

Libova, I

Fässler, R

Wehland, J

Gertler, FB ^{[1
]}

机构：

[1] MIT, Dept Biol, Cambridge, MA 02139 USA

[2] Univ Lund Hosp, S-22185 Lund, Sweden

[3] Gesell Biotechnol Forsch mbH, D-38124 Braunschweig, Germany

来源：

CELL | 2000年 / 101卷 / 07期

关键词：

D O I：

10.1016/S0092-8674(00)80884-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Ena/VASP proteins have been implicated in cell motility through regulation of the actin cytoskeleton and are found at focal adhesions and the leading edge. Using overexpression, loss-of-function, and inhibitory approaches, we find that Ena/VASP proteins negatively regulate fibroblast motility. A close-dependent decrease in movement is observed when Ena/VASP proteins are overexpressed in fibroblasts. Neutralization or deletion of all Ena/VASP proteins results in increased cell movement. Selective depletion of Ena/VASP proteins from focal adhesions, but not the leading edge, has no effect on motility. Constitutive membrane targeting of Ena/VASP proteins inhibits motility. These results are in marked contrast to current models for Ena/VASP function derived mainly from their role in the actin-driven movement of Listeria monocytogenes.

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页码：717 / 728

页数：12

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