Rapid and high-efficiency generation of mature functional hepatocyte-like cells from adipose-derived stem cells by a three-step protocol

被引：23

作者：

Xu, Fen ^{[1
,2
,3
]}

Liu, Junli ^{[1
,2
,3
]}

Deng, Jie ^{[1
,2
,3
]}

Chen, Xiaolei ^{[1
,2
,3
]}

Wang, Yuan ^{[1
,2
,3
]}

Xu, Pengchao ^{[1
,2
,3
]}

Cheng, Lin ^{[1
,2
,3
]}

Fu, Yanli ^{[1
,2
,3
]}

Cheng, Fuyi ^{[1
,2
,3
]}

Yao, Yunqi ^{[1
,2
,3
]}

Zhang, Yujing ^{[1
,2
,3
]}

Huang, Meijuan ^{[1
,2
,3
,4
]}

Yu, Dechao ^{[1
,2
,3
]}

Wei, Yuquan ^{[1
,2
,3
]}

Deng, Hongxin ^{[1
,2
,3
]}

机构：

[1] Sichuan Univ, West China Hosp, State Key Lab Biotherapy, Chengdu 610041, Sichuan, Peoples R China

[2] Sichuan Univ, West China Hosp, Ctr Canc, Chengdu 610041, Sichuan, Peoples R China

[3] Collaborat Innovat Ctr Biotherapy, Chengdu 610041, Peoples R China

[4] Sichuan Univ, West China Hosp, Ctr Canc, Dept Thorac Oncol, Chengdu 610041, Sichuan, Peoples R China

来源：

STEM CELL RESEARCH & THERAPY | 2015年 / 6卷

关键词：

INSULIN-PRODUCING CELLS; IN-VITRO; DIFFERENTIATION; TISSUE; VIVO; TRANSLATION; THERAPIES; CULTURE;

D O I：

10.1186/s13287-015-0181-3

中图分类号：

Q813 [细胞工程];

学科分类号：

100113 [医学细胞生物学];

摘要：

The generation of functional hepatocytes is a major challenge for regenerative medicine and drug discovery. Here we show a method that facilitates generation of induced functional hepatocytes (iHeps) from adipose-derived stem cells (ADSCs) within 9 days. iHeps express hepatocytic gene programs and display functions characteristic of mature hepatocytes, including cytochrome P450 enzyme activity. Upon transplantation into mice with carbon tetrachloride (CCl4)-induced acute fulminant liver failure, iHeps restore the liver function and prolong survival. The work could contribute to the development of alternative strategies to obtain nonhepatic cell-derived mature hepatocytes with potential for biomedical and pharmaceutical applications.

引用

页数：10

共 36 条

[1]

Hepatocyte differentiation of mesenchymal stem cells from human adipose tissue in vitro promotes hepatic integration in vivo [J].