The genome structure of Pseudomonas putida:: high-resolution mapping and microarray analysis

被引:12
作者
Stjepandic, D
Weinel, C
Hilbert, H
Koo, HL
Diehl, F
Nelson, KE
Tümmler, B
Hoheisel, JD
机构
[1] Deutsch Krebsforschungszentrum, D-69120 Heidelberg, Germany
[2] Hannover Med Sch, Klin Forschergrp, D-30623 Hannover, Germany
[3] QIAGEN GmbH, D-40724 Hilden, Germany
[4] Inst Genom Res, Rockville, MD 20850 USA
关键词
D O I
10.1046/j.1462-2920.2002.00313.x
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
As part of a collaborative project aimed at sequencing and functionally analysing the entire genome of Pseudomonas putida strain KT2440, a physical clone map was produced as an initial resource. To this end, a high-coverage cosmid library was arrayed and ordered by clone hybridizations. Restriction fragments generated by rare-cutting enzymes and plasmids containing the rrn operon and 23S rDNA of Pseudomonas aeruginosa were used as probes and, parts of the cosmids were end-sequenced. This provided the information necessary for merging and comparing the macro-restriction map, cosmid clone order and sequence information, thereby assuring co-linearity of the eventual sequence assembly with the actual genome. A tiling path of clones was selected, from the shotgun clones used for sequencing, for the production of DNA microarrays that represent the entire genome including its non-coding portions.
引用
收藏
页码:819 / 823
页数:5
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