Developmental Validation of the PrepFiler™ Forensic DNA Extraction Kit for Extraction of Genomic DNA from Biological Samples

被引：45

作者：

Brevnov, Maxim G. ^{[1
]}

Pawar, Hemant S. ^{[1
]}

Mundt, Janna ^{[1
]}

Calandro, Lisa M. ^{[1
]}

Furtado, Manohar R. ^{[1
]}

Shewale, Jaiprakash G. ^{[1
]}

机构：

[1] Appl Biosyst Inc, Foster City, CA 94404 USA

来源：

JOURNAL OF FORENSIC SCIENCES | 2009年 / 54卷 / 03期

关键词：

forensic science; DNA extraction; DNA purification; DNA isolation; DNA typing; short tandem repeat profiling; PCR AMPLIFICATION KIT; MULTIPLEX SYSTEM; TWGDAM VALIDATION; STR MULTIPLEX; NUCLEAR-DNA; ASSAY; QUANTIFICATION; CASEWORK;

D O I：

10.1111/j.1556-4029.2009.01013.x

中图分类号：

DF [法律]; D9 [法律]; R [医药、卫生];

学科分类号：

0301 ; 10 ;

摘要：

The PrepFiler (TM) Forensic DNA Extraction Kit enables isolation of genomic DNA from a variety of biological samples. The kit facilitates reversible binding of DNA with magnetic particles resulting in high DNA recovery from samples with very low and high quantities of biological materials: 0.1 and 40 mu L of human blood (donor 2) provided 14 and 2883 ng of DNA, respectively. Following the revised SWGDAM guidelines, performance of the developed method was investigated using different sample types including saliva on swabs, semen stains on cotton fabric, samples exposed to environment, samples with polymerase chain reaction (PCR) inhibitors, blood stains (on denim, cotton cloth, and FTA((R)) paper), and touch evidence-type samples. DNA yields for all samples tested were equal or better than those obtained by both phenol-chloroform extraction and commercial kits tested. DNA obtained from these samples was free of detectable PCR inhibitors. Short tandem repeat profiles were complete, conclusive, and devoid of PCR artifacts.

引用

页码：599 / 607

页数：9

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