Determining the primary sources of fecal pollution using microbial source tracking assays combined with land-use information in the Edwards Aquifer

被引:29
作者
Hinojosa, Jessica [1 ]
Green, Jemima [1 ]
Estrada, Fabiola [1 ]
Herrera, Jonathan [1 ]
Mata, Troy [1 ]
Phan, Duc [1 ]
Pasha, A. B. M. Tanvir [1 ,2 ]
Matta, Akanksha [1 ]
Johnson, Drew [1 ]
Kapoor, Vikram [1 ]
机构
[1] Univ Texas San Antonio, Dept Civil & Environm Engn, San Antonio, TX 78249 USA
[2] North Carolina State Univ, Dept Civil Construct & Environm Engn, Raleigh, NC 27695 USA
基金
美国国家科学基金会;
关键词
Microbial source tracking; Water quality; qPCR; Fecal pollution; Bacteroidales; QUANTITATIVE PCR ASSAYS; GENETIC-MARKERS; RIBOSOMAL-RNA; ESCHERICHIA-COLI; BACTERIA CONTAMINATION; ENVIRONMENTAL WATERS; INDICATOR BACTERIA; SEPTIC SYSTEMS; DRINKING-WATER; BACTEROIDALES;
D O I
10.1016/j.watres.2020.116211
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
The Edwards Aquifer serves as a primary source of drinking water to more than 2 million people in south-central Texas, and as a karst aquifer, is vulnerable to human and animal fecal contamination which poses a serious risk to human and environmental health. A one-year study (Jan 2018 - Feb 2019) was conducted to determine the primary sources of fecal pollution along the Balcones and Leon Creek within the Edwards Aquifer recharge and contributing zones using general (E. coli, enterococci, and universal Bacteriodales) and host-associated (human-, dog-, cow- and chicken/duck-associated Bacteriodales) microbial source tracking (MST) assays. Additionally, sites were classified based on surrounding land use as a potential source predictor and marker levels were correlated with rain events and water quality parameters. Levels for the three general indicators were highest and exhibited similar trends across the sampling sites, suggesting that the sole use of these markers is not sufficient for specific fecal source identification. Among the host-associated markers, highest concentrations were observed for the dog marker (BacCan) in the Leon Creek area and the cow marker (BacCow) in the Balcones Creek area. Additionally, Chicken/Duck-Bac, BacCan and BacCow all exhibited higher concentrations during the spring season and the end of fall/early winter. Relatively lower concentrations were observed for the human-associated markers (HF183 and BacHum), however, levels were higher in the Leon Creek area and highest following rainfall events. Additionally, relatively higher levels in HF183 and BacHum were observed at sites having greater human population and septic tank density and may be attributed to leaks or breaks in these infrastructures. This study is the first to examine and compare fecal contamination at rural and urban areas in the recharge and contributing zones of the Edwards Aquifer using a molecular MST approach targeting Bacteroidales 16S rRNA gene-based assays. The Bacteroidales marker assays, when combined with land use and weather information, can allow for a better understanding of the sources and fluxes of fecal contamination, which can help devise effective mitigation measures to protect water quality. (c) 2020 Elsevier Ltd. All rights reserved.
引用
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页数:11
相关论文
共 71 条
[1]   Persistence and differential survival of fecal indicator bacteria in subtropical waters and sediments [J].
Anderson, ML ;
Whitlock, JE ;
Harwood, VJ .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2005, 71 (06) :3041-3048
[2]  
[Anonymous], 2019, Bexar Appraisal District (Bexar CAD)
[3]  
[Anonymous], 2012, 2012 CENS AGR COUNT
[4]   Short-term microbial release during rain events from on-site sewers and cattle in a surface water source [J].
Astrom, Johan ;
Pettersson, Thomas J. R. ;
Reischer, Georg H. ;
Hermansson, Malte .
JOURNAL OF WATER AND HEALTH, 2013, 11 (03) :430-442
[5]  
AVMA, 2019, US PET OWN STAT
[6]   Fecal indicator dynamics at the watershed scale: Variable relationships with land use, season, and water chemistry [J].
Badgley, Brian D. ;
Steele, Meredith K. ;
Cappellin, Catherine ;
Burger, Julie ;
Jian, Jinshi ;
Neher, Timothy P. ;
Orentas, Megan ;
Wagner, Regan .
SCIENCE OF THE TOTAL ENVIRONMENT, 2019, 697
[7]   Decay of host-associated Bacteroidales cells and DNA in continuous-flow freshwater and seawater microcosms of identical experimental design and temperature as measured by PMA-qPCR and qPCR [J].
Bae, Sungwoo ;
Wuertz, Stefan .
WATER RESEARCH, 2015, 70 :205-213
[8]   Factors Influencing the Persistence of Fecal Bacteroides in Stream Water [J].
Bell, Alyssa ;
Layton, Alice C. ;
McKay, Larry ;
Williams, Dan ;
Gentry, Randy ;
Sayler, Gary S. .
JOURNAL OF ENVIRONMENTAL QUALITY, 2009, 38 (03) :1224-1232
[9]   A PCR assay to discriminate human and ruminant feces on the basis of host differences in Bacteroides-Prevotella genes encoding 16S rRNA [J].
Bernhard, AE ;
Field, KG .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2000, 66 (10) :4571-4574
[10]   Performance of forty-one microbial source tracking methods: A twenty-seven lab evaluation study [J].
Boehm, Alexandria B. ;
Van De Werfhorst, Laurie C. ;
Griffith, John F. ;
Holden, Patricia A. ;
Jay, Jenny A. ;
Shanks, Orin C. ;
Wang, Dan ;
Weisberg, Stephen B. .
WATER RESEARCH, 2013, 47 (18) :6812-6828