Rna14p, a component of the yeast nuclear Cleavage/Polyadenylation Factor I, is also localised in mitochondria

被引:12
作者
Rouillard, JM [1 ]
Brendolise, C [1 ]
Lacroute, F [1 ]
机构
[1] CNRS, Ctr Genet Mol, F-91198 Gif Sur Yvette, France
来源
MOLECULAR AND GENERAL GENETICS | 2000年 / 262卷 / 06期
关键词
Saccharomyces cerevisiae; 3 '-end mRNA processing; mitochondria; bi-functional protein;
D O I
10.1007/PL00008653
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
RNA14 was identified as a gene involved in premessenger RNA cleavage and polyadenylation. These processing steps take place in the nucleus, but the Rna14p protein is distributed in both the nucleus and the cytoplasm. By subcellular fractionation, we show here that the cytoplasmic fraction is localised in the mitochondria. In order to understand the role played by Rna14p in mitochondria, we have searched fbr new thermosensitive alleles of RNA14. We isolated thirteen new mutants. Some of them are deficient in mRNA cleavage and polyadenylation at the restrictive temperature - like the first mutant identified (rna14-1). However, others do not appear to be impaired in any of the steps in RNA metabolism investigated, nor do they appear to be involved in the replication or expression of mitochondrial,DNA or in respiration. The localisation data strongly suggest that, besides an essential function in mRNA polyadenylation, the Rna14p protein has a non essential function in mitochondrial metabolism.
引用
收藏
页码:1103 / 1112
页数:10
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