Characterization of bacterial diversity in Pulque, a traditional Mexican alcoholic fermented beverage, as determined by 16S rDNA analysis

被引：30

作者：

Escalante, A ^{[1
]}

Rodríguez, ME ^{[1
]}

Martínez, A ^{[1
]}

López-Munguía, A ^{[1
]}

Bolívar, F ^{[1
]}

Gosset, G ^{[1
]}

机构：

[1] Univ Nacl Autonoma Mexico, Inst Biotecnol, Dept Ingn Celular & Biocatalisis, Cuernavaca 62250, Morelos, Mexico

来源：

FEMS MICROBIOLOGY LETTERS | 2004年 / 235卷 / 02期

关键词：

Pulque; bacterial diversity; 16S rDNA sequence analysis;

D O I：

10.1016/j.femsle.2004.04.045

中图分类号：

Q93 [微生物学];

学科分类号：

071005 [微生物学]; 100705 [微生物与生化药学];

摘要：

The bacterial diversity in pulque, a traditional Mexican alcoholic fermented beverage, was studied in 16S rDNA clone libraries from three pulque samples. Sequenced clones identified as Lactobacillus acidophilus, Lactobacillus strain ASF360, L. kefir, L. acetotolerans, L. hilgardii, L. plantarum, Leuconostoc pseudomesenteroides, Microbacterium arborescens, Flavobacterium johnsoniae, Acetobacter pomorium, Gluconobacter oxydans, and Hafnia alvei, were detected for the first time in pulque. Identity of 16S rDNA sequenced clones showed that bacterial diversity present among pulque samples is dominated by Lactobacillus species (80.97%). Seventy-eight clones exhibited less than 95% of relatedness to NCBI database sequences, which may indicate the presence of new species in pulque samples. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：273 / 279

页数：7

共 22 条

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Molecular microbial diversity of an agricultural soil in Wisconsin [J].