Analysis of steroids in environmental water samples using solid-phase extraction and ion-trap gas chromatography-mass spectrometry and gas chromatography-tandem mass spectrometry

被引：98

作者：

Kelly, C ^{[1
]}

机构：

[1] Ctr Environm Fisheries & Aquaculture Sci, CEFAS Burnham Lab, Burnham On Crouch CM0 8HA, Essex, England

来源：

JOURNAL OF CHROMATOGRAPHY A | 2000年 / 872卷 / 1-2期

关键词：

water analysis; environmental analysis; steroids;

D O I：

10.1016/S0021-9673(99)01261-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This paper describes an improved method for the extraction and determination of three steroids, oestrone, 17 beta-oestradiol, and the synthetic contraceptive steroid 17 alpha-ethinyloestradiol in aqueous matrices. Samples of wastewater and environmental water were spiked with internal standards, comprising isotopically labelled analogues of the steroids to be determined. The samples were extracted using solid-phase extraction disks and the extracts were then derivatized to form tert.-butyl-dimethylsilyl derivatives. The derivatised steroids were determined in the final extracts by CC-MS or GC-MS-MS allowing an operational detection limit for each steroid in effluent samples of 1 ng l(-1). Crown copyright (C) 2000 Published by Elsevier Science BN. All rights reserved.

引用

页码：309 / 314

页数：6

共 6 条

[1] Identification of estrogenic chemicals in STW effluent. 1. Chemical fractionation and in vitro biological screening [J].