Increased enterocyte production in gnotobiotic rats mono-associated with Lactobacillus rhamnosus GG

被引:72
作者
Banasaz, M
Norin, E
Holma, R
Midtvedt, T
机构
[1] Karolinska Inst, Dept Cell & Mol Biol, Lab Med Microbial Ecol, S-17177 Stockholm, Sweden
[2] Karolinska Inst, Ctr Microbiol & Tumor Biol, S-17177 Stockholm, Sweden
[3] Univ Helsinki, Inst Biomed & Pharmacol, Biomedicum Helsinki, Helsinki, Finland
关键词
D O I
10.1128/AEM.68.6.3031-3034.2002
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
There is increasing scientific and commercial interest in using beneficial microorganisms (i.e., probiotics) to enhance intestinal health. Of the numerous microbial strains examined, Lactobacillus rhamnosus GG has been most extensively studied. Daily intake of L. rhamnosus GG shortens the course of rotavirus infection by mechanisms that have not been fully elucidated. Comparative studies with germfree and conventional rats have shown that the microbial status of an animal influences the intestinal cell kinetics and morphology. The present study was undertaken to study whether establishment of L. rhamnosus GG as a mono-associate in germfree rats influences intestinal cell kinetics and morphology. L. rhamnosus GG was easily established in germfree rats. After 3 days of mono-association, the rate of mitoses in the upper part of the small intestine (jejunum 1) increased as much as 14 and 22% compared to the rates in germfree and conventional counterparts, respectively. The most striking alteration in morphology was an increase in the number of cells in the villi. We hypothesis that the compartmentalized effects of L. rhamnosus GG may represent a reparative event for the mucosa.
引用
收藏
页码:3031 / 3034
页数:4
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