The IL-6-STAT3 axis mediates a reciprocal crosstalk between cancer-derived mesenchymal stem cells and neutrophils to synergistically prompt gastric cancer progression

被引:179
作者
Zhu, Q. [1 ]
Zhang, X. [1 ]
Zhang, L. [1 ]
Li, W. [2 ]
Wu, H. [2 ]
Yuan, X. [1 ]
Mao, F. [1 ]
Wang, M. [1 ]
Zhu, W. [1 ]
Qian, H. [1 ]
Xu, W. [1 ,3 ]
机构
[1] Jiangsu Univ, Sch Med Sci & Lab Med, Zhenjiang 212013, Jiangsu, Peoples R China
[2] First Peoples Hosp Lianyungang, Dept Cent Lab, Lianyungang, Jiangsu, Peoples R China
[3] Jiangsu Univ, Affiliated Hosp, Zhenjiang 212013, Jiangsu, Peoples R China
基金
中国国家自然科学基金;
关键词
TUMOR MICROENVIRONMENT; ACTIVATION; MACROPHAGES; METASTASIS; MODEL;
D O I
10.1038/cddis.2014.263
中图分类号
Q2 [细胞生物学];
学科分类号
071013 [干细胞生物学];
摘要
Emerging evidence indicate that mesenchymal stem cells (MSCs) affect tumor progression by reshaping the tumor microenvironment. Neutrophils are essential component of the tumor microenvironment and are critically involved in cancer progression. Whether the phenotype and function of neutrophils is influenced by MSCs is not well understood. Herein, we investigated the interaction between neutrophils and gastric cancer-derived MSCs (GC-MSCs) and explored the biological role of this interaction. We found that GC-MSCs induced the chemotaxis of neutrophils and protected them from spontaneous apoptosis. Neutrophils were activated by the conditioned medium from GC-MSCs with increased expression of IL-8, TNF alpha, CCL2, and oncostatin M (OSM). GC-MSCs-primed neutrophils augmented the migration of gastric cancer cells in a cell contact-dependent manner but had minimal effect on gastric cancer cell proliferation. In addition, GC-MSCs-primed neutrophils prompted endothelial cells to form tube-like structure in vitro. We demonstrated that GC-MSCs stimulated the activation of STAT3 and ERK1/2 pathways in neutrophils, which was essential for the functions of activated neutrophils. We further revealed that GC-MSCs-derived IL-6 was responsible for the protection and activation of neutrophils. In turn, GC-MSCs-primed neutrophils induced the differentiation of normal MSCs into cancer-associated fibroblasts (CAFs). Collectively, our results suggest that GC-MSCs regulate the chemotaxis, survival, activation, and function of neutrophils in gastric cancer via an IL-6-STAT3-ERK1/2 signaling cascade. The reciprocal interaction between GC-MSCs and neutrophils presents a novel mechanism for the role of MSCs in remodeling cancer niche and provides a potential target for gastric cancer therapy.
引用
收藏
页码:e1295 / e1295
页数:11
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