Identification of transcriptional effects of ethynyl oestradiol in male plaice (Pleuronectes platessa) by suppression subtractive hybridisation and a nylon macroarray

被引：12

作者：

Brown, M

Davies, IM

Moffat, CF

Robinson, C

Redshaw, J

Craft, JA

机构：

[1] Glasgow Caledonian Univ, Glasgow G4 0BA, Lanark, Scotland

[2] Fisheries Res Serv, Marine Lab, Aberdeen, Scotland

[3] Scottish Environm Protect Agcy, E Kilbride, Lanark, Scotland

来源：

MARINE ENVIRONMENTAL RESEARCH | 2004年 / 58卷 / 2-5期

关键词：

Pleuronectes platessa; suppression subtractive hybridisation; ethynyl oestradiol; endocrine disruption; transcription profiling;

D O I：

10.1016/j.marenvres.2004.03.045

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Suppression subtractive hybridisation (SSH) was used to generate cDNA libraries representing genes differentially expressed in response to ethynyl oestradiol (EE2) exposure in liver from male plaice (Pleuronectes platessa) previously analysed for vitellogenin (VTG) induction. Characterisation of the cDNA clones identified many as VTG (2 genes) and zona radiata proteins (ZRP) (3 genes), but 40 encoded other proteins, with more than half cryptic. Further analysis identified 85 non-redundant clones suitable for array on nylon membrane. Radiolabelled cDNAs were prepared from hepatic mRNA from EE2 treated plaice (0 and 21 days) and hybridised with the arrayed clones. Analysis of the data showed that 11/17 novel, 21/22 VTG, 13/14 ZRP, 2/2 liver aspartic proteinase (LAP) and 8/10 other mRNAs were up-regulated by EE2 exposure. Crown copyright (C) 2004 Published by Elsevier Ltd. All rights reserved.

引用

页码：559 / 563

页数：5

共 3 条

[1] Suppression subtractive hybridization: A method for generating differentially regulated or tissue-specific cDNA probes and libraries [J].