alpha(1)-proteinase inhibitor in gingival crevicular fluid of humans with adult periodontitis: Serpinolytic inhibition by doxycycline

被引:33
作者
Lee, HM
Golub, LM
Chan, D
Leung, M
Schroeder, K
Wolff, M
Simon, S
Crout, R
机构
[1] SUNY STONY BROOK,SCH DENT MED,DEPT PERIODONT,STONY BROOK,NY 11794
[2] SUNY STONY BROOK,SCH DENT MED,DEPT GEN DENT,STONY BROOK,NY 11794
[3] SUNY STONY BROOK,SCH DENT MED,DEPT PATHOL,STONY BROOK,NY 11794
[4] SUNY STONY BROOK,SCH MED,DEPT ORAL BIOL & PATHOL,STONY BROOK,NY 11794
[5] SUNY STONY BROOK,SCH MED,DEPT PERIODONT,STONY BROOK,NY 11794
[6] SUNY STONY BROOK,SCH MED,DEPT GEN DENT,STONY BROOK,NY 11794
[7] SUNY STONY BROOK,SCH MED,DEPT PATHOL,STONY BROOK,NY 11794
[8] SUNY COLL OLD WESTBURY,DEPT CHEM,OLD WESTBURY,NY 11568
[9] W VIRGINIA UNIV,DEPT PERIODONTOL,MORGANTOWN,WV
关键词
alpha(1)-proteinase inhibitor; gingival crevicular fluid; adult periodontitis; doxycycline;
D O I
10.1111/j.1600-0765.1997.tb01377.x
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
The serum protein, alpha(1)-proteinase inhibitor (alpha(1)-PI), defends the host against serine proteinases, e.g. PMN elastase. Using a rabbit anti-serum against human alpha(1)-PI, this protein in GCF was quantified from a standard curve constructed from dot-blot analysis and characterized by Western blot. GCF was collected on filter paper strips from healthy (H), gingivitis (G) and adult periodontitis (AP) patients, then extracted with Tris/NaCl/CaCl2 buffer, pH 7.6. alpha(1)-PI concentration increased with G and was highest in AP subjects. H sites only showed intact alpha(1)-PI (52 kDa); no degradation fragments (48 kDa) were detected. In G and AP subjects, alpha(1)-PI degradation fragments were seen in 17% and 71% of GCF samples, respectively. Both collagenase and alpha(1)-PI-degrading activities in GCF increased with severity of inflammation (GCF flow). Moreover, the alpha(1)-PI degrading (or serpinolytic) activity was characterized as a matrix metalloproteinase, probably collagenase, based on its in vitro response to a panel of different proteinase inhibitors including doxycycline. We propose: (1) that collagenase promotes periodontal breakdown not only by degrading collagen, but also by depleting alpha(1)-PI regulation of elastase and other serine-proteinases, thereby favoring a broader attack on extracellular matrix (ECM) constituents, and (2) based on a recent longitudinal double-blind study using the techniques described above for alpha(1)-PI analysis, that low-dose doxycycline administration to humans with adult periodontitis can inhibit this broad cascade of ECM degradation.
引用
收藏
页码:9 / 19
页数:11
相关论文
共 56 条
[1]   THE ABILITY OF GINGIVAL CREVICULAR FLUID ACUTE PHASE PROTEINS TO DISTINGUISH HEALTHY, GINGIVITIS AND PERIODONTITIS SITES [J].
ADONOGIANAKI, E ;
MOONEY, J ;
KINANE, DF .
JOURNAL OF CLINICAL PERIODONTOLOGY, 1992, 19 (02) :98-102
[2]   LONGITUDINAL EVALUATION OF ELASTASE AS A MARKER FOR THE PROGRESSION OF PERIODONTITIS [J].
ARMITAGE, GC ;
JEFFCOAT, MK ;
CHADWICK, DE ;
TAGGART, EJ ;
NUMABE, Y ;
LANDIS, JR ;
WEAVER, SL ;
SHARP, TJ .
JOURNAL OF PERIODONTOLOGY, 1994, 65 (02) :120-128
[3]   THE INHIBITORY COMPLEX OF HUMAN ALPHA-1-PROTEINASE INHIBITOR AND HUMAN-LEUKOCYTE ELASTASE IS A NEUTROPHIL CHEMOATTRACTANT [J].
BANDA, MJ ;
RICE, AG ;
GRIFFIN, GL ;
SENIOR, RM .
JOURNAL OF EXPERIMENTAL MEDICINE, 1988, 167 (05) :1608-1615
[4]  
Bauer J, 1988, Adv Exp Med Biol, V240, P425
[5]   A COLLAGENASE ASSAY USING [METHYL]COLLAGEN-H-3 [J].
BHATNAGAR, R ;
DECKER, K .
JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS, 1981, 5 (03) :147-152
[6]   MATRIX METALLOPROTEINASES - A REVIEW [J].
BIRKEDALHANSEN, H ;
MOORE, WGI ;
BODDEN, MK ;
WINDSOR, LJ ;
BIRKEDALHANSEN, B ;
DECARLO, A ;
ENGLER, JA .
CRITICAL REVIEWS IN ORAL BIOLOGY & MEDICINE, 1993, 4 (02) :197-250
[7]   PROTEASE-ANTIPROTEASE IMBALANCE IN THE LUNGS OF CHILDREN WITH CYSTIC-FIBROSIS [J].
BIRRER, P ;
MCELVANEY, NG ;
RUDEBERG, A ;
SOMMER, CW ;
LIECHTIGALLATI, S ;
KRAEMER, R ;
HUBBARD, R ;
CRYSTAL, RG .
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 1994, 150 (01) :207-213
[8]   INHIBITION OF NEUTROPHIL SUPEROXIDE PRODUCTION BY HUMAN PLASMA ALPHA-1-ANTITRYPSIN [J].
BUCURENCI, N ;
BLAKE, DR ;
CHIDWICK, K ;
WINYARD, PG .
FEBS LETTERS, 1992, 300 (01) :21-24
[9]   INVITRO SUPPRESSION OF SERUM ELASTASE-INHIBITORY CAPACITY BY REACTIVE OXYGEN SPECIES GENERATED BY PHAGOCYTOSING POLYMORPHONUCLEAR LEUKOCYTES [J].
CARP, H ;
JANOFF, A .
JOURNAL OF CLINICAL INVESTIGATION, 1979, 63 (04) :793-797
[10]  
CIMASONI G, 1983, CREVICULAR FLUID UPD