Simian immunodeficiency virus (SIV)-specific CD8(+) cytotoxic T lymphocyte responses of naive and vaccinated cynomolgus macaques infected with SIVmac32H(J5): quantitative analysis by in vitro antigenic stimulation

被引:9
作者
Geretti, AM
Hulskotte, EGJ
Dings, MEM
vanBaalen, CA
vanAmerongen, G
Osterhaus, ADME
机构
[1] ERASMUS UNIV ROTTERDAM,INST VIROL,NL-3000 DR ROTTERDAM,NETHERLANDS
[2] NATL INST PUBL HLTH & ENVIRONM PROTECT,CENT ANIM LAB,NL-3720 BA BILTHOVEN,NETHERLANDS
关键词
animal models; protective immunity; recombinant antigens;
D O I
10.1016/S0264-410X(97)00029-7
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Detailed analyses of simian immunodeficiency virus (SN)-specific cytotoxic T lymphocyte (CTL) responses bz vaccinated and infected macaques may help to clarify the role of CTL immunity in protection against lentiviruses, Here, the optimal conditions for the measurement of SN Gag-specific CTL were investigated by bulk and limiting dilution assays of peripheral blood mononuclear cells (PBMC) from naive and vaccinated cynomolgus macaques (Macaca fascicularis) infected with SIVmac32H(J5). In vitro restimulation was generally required for CTL detection. Selective activation of CD8(+) and MHC-restricted SN Gag-specific CTL was induced by stimulation with autologous para-formaldehyde-fixed B-lymphoblastoid cell lines infected with a recombinant vaccinia virus expressing SN Gag, Applied to limiting dilution assays, antigenic stimulation reproducibly demonstrated SIV Gag-specific CTL precursors (CTLp) in PBMC of all animals studied including those lacking significant responses in standard bulk CTL assays. (C) 1997 Elsevier Science Ltd.
引用
收藏
页码:1269 / 1275
页数:7
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