Analysis of hydrophilic metabolites in physiological fluids by HPLC-MS using a silica hydride-based stationary phase

被引：56

作者：

Pesek, Joseph J. ^{[1
]}

Matyska, Maria T. ^{[1
]}

Loo, Joseph A. ^{[2
,3
]}

Fischer, Steven M. ^{[4
]}

Sana, Theodore R. ^{[4
]}

机构：

[1] San Jose State Univ, Dept Chem, San Jose, CA 95192 USA

[2] Univ Calif Los Angeles, David Geffen Sch Med, Dept Biol Chem, Los Angeles, CA 90095 USA

[3] Univ Calif Los Angeles, Dept Chem & Biochem, Los Angeles, CA 90024 USA

[4] Agilent Technol, Metabol Lab, Santa Clara, CA USA

来源：

JOURNAL OF SEPARATION SCIENCE | 2009年 / 32卷 / 13期

关键词：

Aqueous normal-phase; LC-MS; Metabolomics; Polar analytes; Saliva; Urine; MASS-SPECTROMETRIC METHOD; LIQUID-CHROMATOGRAPHY; SEPARATION; RETENTION; URINE;

D O I：

10.1002/jssc.200900270

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Aqueous normal-phase chromatography was used for the analysis of metabolites in human saliva and urine samples. The column was packed with a silica hydride type separation material. Several gradients were tested with different mobile phase additives in order to produce retention for amino acids, small organic acids, and carbohydrates. Detection was done by TOF MS. In some cases the relative concentration levels of various metabolites in human saliva were compared for normal patients and patients with pancreatic cancer or pancreatitis. The reproducibility of retention of individual metabolites in these complex matrices was tested for several compounds.

引用

页码：2200 / 2208

页数：9

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