Immunosuppressive activity of recombinant ILT3

被引：10

作者：

Vlad, George ^{[1
]}

Liu, Zhuoru ^{[1
]}

Zhang, Qing-Yin ^{[1
]}

Cortesini, Raffaello ^{[1
]}

Suciu-Foca, Nicole ^{[1
]}

机构：

[1] Columbia Univ, Dept Pathol, New York, NY 10032 USA

来源：

INTERNATIONAL IMMUNOPHARMACOLOGY | 2006年 / 6卷 / 13-14期

关键词：

CD8(+) T suppressor cells; tolerogenic APC; membrane ILT3; soluble ILT3;

D O I：

10.1016/j.intimp.2006.07.017

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Tolerogenic antigen presenting cells (APC) are characterized by high expression of the inhibitory receptors ILT3 and ILT4. We have engineered ILT3 and ILT4 cytoplasmic deletion mutants (ILT3delta and ILT4delta), which were transfected in the dendritic-like cell line KG 1, to investigate ILT3 and ILT4's capacity to signal extracellularly. KG1.ILT3delta, similar to untruncated ILT3, inhibits T cell responses such as proliferation and cell-mediated cytotoxicity. In contrast, KG1.ILT4delta lost the suppressive activity of untruncated ILT4. This indicates that the inhibitory function of ILT4 relies entirely on the cytoplasmic region containing ITIM motifs. We further demonstrated that recombinant soluble ILT3 inhibits T helper and cytotoxic function while inducing the differentiation of CD8(+) Ts cells. Hence, Ts modulated APC function inducing inhibitory receptors, which in turn elicit the generation of Ts. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：1889 / 1894

页数：6

共 23 条

[1] Interleukin 10 regulates cell surface and soluble LIR-2 (CD85d) expression on dendritic cells resulting in T cell hyporesponsiveness in vitro [J].