A cryptic type I polyketide synthase (cpk) gene cluster in Streptomyces coelicolor A3(2)

被引：71

作者：

Pawlik, Krzysztof

Kotowska, Magdalena

Chater, Keith F.

Kuczek, Katarzyna

Takano, Eriko

机构：

[1] Univ Groningen, Groningen Biomol Sci & Biotechnol, Dept Microbial Physiol, NL-9751 NN Haren, Netherlands

[2] Polish Acad Sci, Inst Immunol & Expt Therapy, PL-53114 Wroclaw, Poland

[3] John Innes Inst, Norwich NR4 7UH, Norfolk, England

来源：

ARCHIVES OF MICROBIOLOGY | 2007年 / 187卷 / 02期

关键词：

Streptomyces; polyketide biosynthesis; post-polyketide modifications; antibiotic biosynthesis; COMPLETE GENOME SEQUENCE; SECONDARY METABOLISM; BIOCHEMICAL-CHARACTERIZATION; ACYLTRANSFERASE DOMAINS; SUBSTRATE-SPECIFICITY; KETOREDUCTASE DOMAINS; ANTIBIOTIC PRODUCTION; ENZYMATIC DOMAINS; ACYL-COENZYME; BIOSYNTHESIS;

D O I：

10.1007/s00203-006-0176-7

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The chromosome of Streptomyces coelicolor A3(2), a model organism for the genus Streptomyces, contains a cryptic type I polyketide synthase (PKS) gene cluster which was revealed when the genome was sequenced. The ca. 54-kb cluster contains three large genes, cpkA, cpkB and cpkC, encoding the PKS subunits. In silico analysis showed that the synthase consists of a loading module, five extension modules and a unique reductase as a terminal domain instead of a typical thioesterase. All acyltransferase domains are specific for a malonyl extender, and have a B-type ketoreductase. Tailoring and regulatory genes were also identified within the gene cluster. Surprisingly, some genes show high similarity to primary metabolite genes not commonly identified in any antibiotic biosynthesis cluster. Using western blot analysis with a PKS subunit (CpkC) antibody, CpkC was shown to be expressed in S. coelicolor at transition phase. Disruption of cpkC gave no obvious phenotype.

引用

页码：87 / 99

页数：13

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