Inhibition of Saccharomyces cerevisiae by combination of hydrostatic pressure and monoterpenes

Using a pressure of 1800 kg/cm, and 150 mu g/mL of alpha-terpinene for 1 hr at 25 degrees C, growth reduction of 6 log cycles after incubation at 30 degrees C/24h, was found with S. cerevisiae. Without pressurization, S. cerevisiae was resistant to 300 mu g/mL and 600 mu g/mL alpha-terpinene but was sensitive to 1250 mu g/mL. Reduction of 3 log cycles in viable cell counts was found when cells were exposed to pressure (1800 kg/cm(2)) and -(+)-limonene (200, 400 and 2,000 mu g/mL).