Angiogenic potential difference between two types of endothelial progenitor cells from human umbilical cord blood

被引：51

作者：

Duan, Hua-Xin ^{[1
]}

Cheng, La-Mei ^{[1
]}

Jian-Wang ^{[1
]}

Hu, Liang-Shan ^{[1
]}

Lu, Guang-Xiu ^{[1
]}

机构：

[1] Cent S Univ, Natl Ctr Human Stem Cell Res & Engn, Inst Human Reprod & Stem Cell Engn, Changsha 410078, Hunan, Peoples R China

来源：

CELL BIOLOGY INTERNATIONAL | 2006年 / 30卷 / 12期

基金：

美国国家科学基金会; 中国国家自然科学基金;

关键词：

endothelial progenitor cells; ischemia; angiogenesis; cell transplantation;

D O I：

10.1016/j.cellbi.2006.08.002

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 [细胞生物学]; 090102 [作物遗传育种];

摘要：

The hierarchy of endothelial progenitor cells (EPCs) in human umbilical cord blood has been disclosed. In this study we compare, for the first time, the angiogenic potential difference between two types of EPCs. We cultured mononuclear cells (MNCs) isolated from human umbilical cord blood using endothelial cell-conditioned medium and obtained two types of EPCs, referred to as circulating angiogenic cells (CACs) and high proliferative potential endothelial progenitor cells (HPP-EPCs). Both types of cells possess characteristics of EPCs, including expressing CD31, VE-cadherin, KDR and von Willebrand factor, uptake of Ac-LDL and binding to lectin. However, unlike CACs, which express CD 14 but not CD133, HPP-EPCs express CD133 but not CD14. Also, unlike CACs, HPP-EPCs display stronger proliferation and clonogenic potential in vitro and show stronger ability to promote vascular growth in the hind-limb model of ischemia in mice (BALB/C-nu) in vivo. (c) 2006 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.

引用

页码：1018 / 1027

页数：10

共 24 条

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