Production, Characterization, and Properties of Sophorolipids from the Yeast Candida bombicola using a Low-cost Fermentative Medium

被引：137

作者：

Daverey, Achlesh ^{[1
]}

Pakshirajan, Kannan ^{[1
]}

机构：

[1] Indian Inst Technol, Dept Biotechnol, Gauhati 781039, India

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 2009年 / 158卷 / 03期

关键词：

Sophorolipids; Biosurfactant; Fermentation; Candida bombicola; Sugarcane molasses; SOY MOLASSES; BIOSURFACTANT; SOPHOROSE; OIL; BIOSYNTHESIS;

D O I：

10.1007/s12010-008-8449-z

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

The yeast Candida bombicola produces biosurfactant with properties akin to those of sophorolipid (SL) group of compounds. In the present work, the yeast was shown to produce 63.7 g l(-1) SL when grown on a cheap fermentative medium containing sugarcane molasses, yeast extract, urea, and soybean oil. The partially purified SL was characterized and confirmed by Fourier-transform infrared (FT-IR) spectroscopy, H-1 and C-13 nuclear magnetic resonance (NMR) and liquid chromatography-in ass spectroscopy (LC-MS) analysis. The critical micelle concentration (CMC) and minimum surface tension of the produced SL in aqueous Solution were found to be 59.43 mg l(-1) and 34.15 m Nm(-1), respectively. The emulsification activity and stability with kerosene oil and organic solvents viz. xylene, benzene, and hexadecane were also tested using the produced SL, which yielded better results compared to those reported in the literature.

引用

页码：663 / 674

页数：12

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