Sensing phosphatase activity by using gold nanoparticles

被引：244

作者：

Choi, Yongdoo ^{[1
]}

Ho, Nan-Hui ^{[1
]}

Tung, Ching-Hsuan ^{[1
]}

机构：

[1] Harvard Univ, Massachusetts Gen Hosp, Sch Med, Ctr Mol Imaging Res, Charlestown, MA 02129 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2007年 / 46卷 / 05期

关键词：

aggregation; assays; charge interaction; nanoparticles; phosphatases;

D O I：

10.1002/anie.200603735

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

To change your colors: A colorimetric enzyme assay based on peptide-induced aggregation of gold nanoparticles (Au-NPs) has been developed to sense phosphatase activity. Once the phosphate group is eliminated by alkaline phosphatase, peptide-induced Au-NP aggregation is triggered, resulting in a significant change in absorbance at 650 nm. (Figure Presented). © 2007 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：707 / 709

页数：3

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