MAP kinase-dependent, NF-κB-independent regulation of inhibitor of apoptosis protein genes by TNF-α

被引:24
作者
Furusu, Akira
Nakayama, Kenji
Xu, Qihe
Konta, Tsuneo
Kitamura, Masanori [1 ]
机构
[1] Univ Yamanashi, Dept Mol Signaling, Interdisciplinary Grad Sch Med & Engn, Yamanashi 4093898, Japan
[2] Nagasaki Univ, Sch Med, Div Nephrol, Dept Internal Med 2, Nagasaki 852, Japan
[3] Tohoku Univ, Grad Sch Med, Div Nephrol Endocrinol & Vasc Med, Sendai, Miyagi 980, Japan
[4] UCL Royal Free & Univ Coll Med Sch, Div Med, Dept Biochem & Mol Biol, London, England
[5] Yamagata Univ, Sch Med, Dept Internal Med 1, Yamagata 99023, Japan
关键词
D O I
10.1002/jcp.20881
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The inhibitor of apoptosis protein (IAP) family of molecules regulates apoptotic processes triggered by various stimuli. However, the mechanisms involved in the regulation of the IAP genes are not fully understood. In this report, we examined roles of nuclear factor-kappa B (NF-kappa B) and mitogen-activated protein (MAP) kinases in tumor necrosis factor-alpha (TNF-alpha)-inducecd expression of IAP genes. In human endothelial cells, TNF-a induced c-IAP1 and c-AP2, but riot XIAP and TIAP/Survivin, at the transcriptional level. Inactivation of NF-kappa B by overexpression of a super-repressor Mutant of I kappa B alpha did not affect the induction of IAPs by TNF-alpha. In contrast, extracellular signal-regulated kinase, p38 MAP kinase, and C-Jun N-terminal kinase were activated after stimulation with TNF-alpha, and inhibition of each kinase by PD098059, SB203580, curcumin, or SP600125 Substantially attenuated the TNF-alpha-induced c-IAP1 and c-IAP2 expression. To examine whether the MAP kinases-mediated induction of IAPs contributes to survival of TNF-alpha-exposed cells, cells were pretreated with MAP kinase inhibitors and stimulated with TNF-alpha. Treatment with kinase inhibitors alone did riot induce apoptosis but enhanced markedly TNF-alpha-triggered apoptosis. Furthermore, overexpression of either c-IAP1 or c-IAP2 diminished the apoptosis-promoting effects of MAP kinase inhibitors. These data indicated that TNF-alpha induced expression of c-IAP1 and c-IAP2 via MAP kinases, but riot via NF-kappa B, and that MAP kinases participated in the inhibition of apoptosis by induction of c-IAPs in TNF-alpha-stimulated endothelial cells.
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收藏
页码:703 / 710
页数:8
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