Development of indirect enzyme-linked immunosorbent assay with nucleoprotein as antigen for detection and quantification of antibodies against avian influenza virus

被引：56

作者：

Wu, R. ^{[1
]}

Hu, S. ^{[1
]}

Xiao, Y. ^{[1
]}

Li, Z. ^{[1
]}

Shi, D. ^{[1
]}

Bi, D. ^{[1
]}

机构：

[1] Huazhong Agr Univ, Lab Anim Microbiol & Immunol, State Key Lab Agr Microbiol, Coll Vet Med, Wuhan, Peoples R China

来源：

VETERINARY RESEARCH COMMUNICATIONS | 2007年 / 31卷 / 05期

关键词：

avian influenza virus; full-length nucleoprotein; NP-ELISA; detection and quantification of antibodies;

D O I：

10.1007/s11259-007-3510-x

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Avian influenza (AI) is a serious infectious disease caused by avian influenza virus (AIV) belonging to type A Orthomyxovirus. In the present study, we developed an indirect enzyme-linked immunosorbent assay (ELISA) employing E. coli-expressed full-length nucleoprotein (NP) of H9N2 avian influenza virus for the detection and quantification of antibodies against AIV nucleoprotein. The NP-ELISA was compared with the AI agar gel propagation (AGP) test, haemagglutination inhibition (HI) test, and IDEXX-FlockChek ELISA using 263 sera. The NP-ELISA was significantly more sensitive than the AGP and HI tests, and showed 96.2% agreement ratio with IDEXX-FlockChek ELISA. With results obtained using the NP-ELISA, an ELISA titre (ET) prediction equation, with which the ELISA titres of a flock or individual chickens can be determined, was derived from a positive/negative (P/N) ratio standard curve. The NP-ELISA enables an alternative rapid serological diagnosis and is suitable for influenza A antibody screening, especially in species that harbour several influenza subtypes.

引用

页码：631 / 641

页数：11

共 21 条

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