Determination of parasitic load in different tissues of murine toxoplasmosis after immunization by excretory-secretory antigens using Real time QPCR

被引：23

作者：

Daryani, Ahmad ^{[1
,2
]}

Sharif, Mehdi ^{[1
,2
]}

Dadimoghaddam, Yousef ^{[1
,2
]}

Souteh, Mohammad Bagher Hashemi ^{[3
]}

Ahmadpour, Ehsan ^{[1
,2
]}

Khalilian, Alireza ^{[4
]}

Sarvi, Shahabeddin ^{[1
,2
]}

Farazmand, Touraj ^{[5
]}

Kalani, Hamed ^{[2
]}

Rasouli, Mehdi ^{[3
]}

机构：

[1] Mazandaran Univ Med Sci, Toxoplasmosis Res Ctr, Sari, Iran

[2] Mazandaran Univ Med Sci, Parasitol & Mycol Dept, Sari Med Sch, Sari, Iran

[3] Mazandaran Univ Med Sci, Fac Med, Dept Biochem Biophys & Genet, Sari, Iran

[4] Mazandaran Univ Med Sci, Dept Biostat, Sari, Iran

[5] Mazandaran Univ Med Sci, Mol & Cell Biol Res Ctr, Sari, Iran

来源：

EXPERIMENTAL PARASITOLOGY | 2014年 / 143卷

关键词：

Toxoplasmosis; Vaccine; Excretory-secretory antigens; Parasite load; QPCR; DNA VACCINE; EXCRETED/SECRETED ANTIGENS; GONDII TACHYZOITES; PERIPHERAL-BLOOD; IMMUNE-RESPONSE; PCR; PROTEINS; MODEL; MICE; ADHESION;

D O I：

10.1016/j.exppara.2014.05.008

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

090105 [作物生产系统与生态工程]; 100103 [病原生物学];

摘要：

Excretory secretory antigens (ESAs) of Toxoplasma gondii are one of the candidates for immunization against toxoplasmosis. For evaluation of immunization, we determined the kinetics of the distribution of Toxoplasma and parasite load in different tissues of mice immunized by ESAs. In this experimental study, 36 mice in case (n = 18) and control (n = 18) groups were immunized with ESAs and PBS, respectively. After 2 weeks, mice were challenged intraperitoneally with Toxoplasma virulent RH strain. Blood and different tissues (brain, spleen, liver, heart, kidney, and muscle) were collected daily after challenge (1, 2,3 and last day before death). Parasite load was calculated using Real time QPCR targeted at the B1 gene. ESAs as vaccine in different tissues showed various effects. However, infected mice which received the vaccine in comparison with control group, displayed a drastically decreasing in parasite burden, in their blood and tissues (P = 0.000). These results indicated that ESAs with reduction of parasite load in different tissues of host could be evaluable candidate for the development of immunization strategies against toxoplasmosis. (C) 2014 Elsevier Inc. All rights reserved.

引用

页码：55 / 59

页数：5

共 33 条

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