Induction of inositol 1,4,5 trisphosphate receptor genes by ionizing radiation

被引:11
作者
Yan, J
Khanna, KK
Lavin, MF
机构
[1] QUEENSLAND INST MED RES, HERSTON, QLD 4029, AUSTRALIA
[2] ROYAL BRISBANE HOSP, BANCROFT CTR, QUEENSLAND INST MED RES, QUEENSLAND CANC FUND RES UNIT, BRISBANE, QLD, AUSTRALIA
[3] UNIV QUEENSLAND, DEPT SURG, BRISBANE, QLD, AUSTRALIA
基金
英国医学研究理事会;
关键词
D O I
10.1080/095530096145544
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
We used differential display, a method designed to amplify partial cDNA sequences from subsets of mRNAs, to identify mRNAs induced by ionizing radiation in human Epstein Barr Virus (EBV)-transformed lymphoblastoid cells. Increased expression of a cDNA corresponding to the inositol 1,4,5 trisphosphate receptor (InsP(3)R) type 1 was observed after exposure of cells to 3Gy gamma-rays. This was confirmed by Northern blot analysis. The increase in mRNA for InsP(3)R type 1 was accompanied by a corresponding increase in the level of InsP(3)R type 1 protein as deter-mined by Western blotting. Exposure of cells from patients with the human genetic disorder ataxia-telangiectasia (A-T), characterized by hypersensitivity to ionizing radiation, failed to change the levels of InsP(3)R type 1 mRNA and, as expected, there was no increase in InsP(3)R type 1 protein in A-T cells in response to radiation exposure. Protein levels for two other InsP(3)Rs, types 2 and 3, were observed to increase in control and A-T cells after exposure to ionizing radiation. The induction of the InsP(3)R type 1, which is primarily located in the endoplasmic reticulum, may play an important role in radiation signal transduction.
引用
收藏
页码:539 / 546
页数:8
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