Nitrate reductase-mediated synthesis of silver nanoparticles from AgNO3

被引：436

作者：

Kumar, S. Anil

Abyaneh, Majid Kazemian

Gosavi, S. W.

Kulkarni, Sulabha K.

Pasricha, Renu

Ahmad, Absar

Khan, M. I. ^{[1
]}

机构：

[1] Natl Chem Lab, Biochem Sci Div, Pune 411008, Maharashtra, India

[2] Pune Univ, DST Unit Nanosci, Dept Phys, Pune 411007, Maharashtra, India

[3] Natl Chem Lab, Mat Chem Div, Pune 411008, Maharashtra, India

来源：

BIOTECHNOLOGY LETTERS | 2007年 / 29卷 / 03期

关键词：

capping peptide; enzymatic method; nitrate reductase; silver nanoparticles;

D O I：

10.1007/s10529-006-9256-7

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Synthesis of silver nanoparticles using alpha-NADPH-dependent nitrate reductase and phytochelatin in vitro has been demonstrated for the first time. The silver ions were reduced in the presence of nitrate reductase, leading to the formation of a stable silver hydrosol 10-25 nm diam. and stabilized by the capping peptide. The nanoparticles were characterized by X-ray diffraction, transmission electron microscopy, X-ray photoelectron spectroscopy and UV-Vis absorption. These studies will help in designing a rational enzymatic strategy for the synthesis of nanomaterials of different chemical composition, shapes and sizes as well as their separation.

引用

页码：439 / 445

页数：7

共 29 条

[1] Extracellular biosynthesis of silver nanoparticles using the fungus Fusarium oxysporum [J].