Archaeal Tuc1/Ncs6 Homolog Required for Wobble Uridine tRNA Thiolation Is Associated with Ubiquitin-Proteasome, Translation, and RNA Processing System Homologs

被引：33

作者：

Chavarria, Nikita E. ^{[1
]}

Hwang, Sungmin ^{[1
]}

Cao, Shiyun ^{[1
]}

Fu, Xian ^{[1
]}

Holman, Mary ^{[1
]}

Elbanna, Dina ^{[1
]}

Rodriguez, Suzanne ^{[1
]}

Arrington, Deanna ^{[1
]}

Englert, Markus ^{[3
]}

Uthandi, Sivakumar ^{[1
]}

Soell, Dieter ^{[3
,4
]}

Maupin-Furlow, Julie A. ^{[1
,2
]}

机构：

[1] Univ Florida, Dept Microbiol & Cell Sci, Gainesville, FL 32611 USA

[2] Univ Florida, Genet Inst, Gainesville, FL 32611 USA

[3] Yale Univ, Dept Mol Biophys & Biochem, New Haven, CT USA

[4] Yale Univ, Dept Chem, New Haven, CT USA

来源：

PLOS ONE | 2014年 / 9卷 / 06期

关键词：

POSTTRANSCRIPTIONAL MODIFICATION; HALOFERAX-VOLCANII; STATISTICAL-MODEL; PROTEINS; TRANSCRIPTION; BIOSYNTHESIS; THIOURIDINE; POSITION; DATABASE; EXOSOME;

D O I：

10.1371/journal.pone.0099104

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

While cytoplasmic tRNA 2-thiolation protein 1 (Tuc1/Ncs6) and ubiquitin-related modifier-1 (Urm1) are important in the 2-thiolation of 5-methoxycarbonylmethyl-2-thiouridine (mcm(5)s(2)U) at wobble uridines of tRNAs in eukaryotes, the biocatalytic roles and properties of Ncs6/Tuc1 and its homologs are poorly understood. Here we present the first report of an Ncs6 homolog of archaea (NcsA of Haloferax volcanii) that is essential for maintaining cellular pools of thiolated tRNA(Lys) UUU and for growth at high temperature. When purified from Hfx. volcanii, NcsA was found to be modified at Lys204 by isopeptide linkage to polymeric chains of the ubiquitin-fold protein SAMP2. The ubiquitin-activating E1 enzyme homolog of archaea (UbaA) was required for this covalent modification. Non-covalent protein partners that specifically associated with NcsA were also identified including UbaA, SAMP2, proteasome activating nucleotidase (PAN)-A/1, translation elongation factor aEF-1 alpha and a beta-CASP ribonuclease homolog of the archaeal cleavage and polyadenylation specificity factor 1 family (aCPSF1). Together, our study reveals that NcsA is essential for growth at high temperature, required for formation of thiolated tRNA(Lys) UUU and intimately linked to homologs of ubiquitin-proteasome, translation and RNA processing systems.

引用

页数：14

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