Talin2 is induced during striated muscle differentiation and is targeted to stable adhesion complexes in mature muscle

被引:61
作者
Senetar, Melissa A. [1 ]
Moncman, Carole L. [1 ]
McCann, Richard O. [1 ]
机构
[1] Univ Kentucky, Coll Med, Dept Mol & Cellular Biochem, Lexington, KY 40536 USA
来源
CELL MOTILITY AND THE CYTOSKELETON | 2007年 / 64卷 / 03期
关键词
talin; muscle differentiation; focal adhesion; costamere; intercalated disk;
D O I
10.1002/cm.20173
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The cytoskeletal protein talin serves as an essential link between integrins and the actin cytoskeleton in several similar, but functionally distinct, adhesion complexes, including focal adhesions, costameres, and intercalated disks. Vertebrates contain two talin genes, TLN1 and TLN2, but the different roles of Talin1 and Talin2 in cell adhesion are unclear. In this report we have analyzed Talin1 and Talin2 in striated muscle. Using isoform-specific antibodies, we found that Talin2 is highly expressed in mature striated muscle. Using mouse C2C12 cells and primary human skeletal muscle myoblasts as models of muscle differentiation, we show that Talin1 is expressed in undifferentiated myoblasts and that Talin2 expression is upregulated during muscle differentiation at both the mRNA and protein levels. We have also identified regulatory sequences that may be responsible for the differential expression of Talin1 and Talin2. Using GFP-tagged Talin1 and Talin2 constructs, we found that GFP-Talin1 targets to focal adhesions while GFP-Talin2 targets to abnormally large adhesions in myoblasts. We also found that ectopic expression of Talin2 in myoblasts, which do not contain appreciable levels of Talin2, dysregulates the actin cytoskeleton. Finally we demonstrate that Talin2, but not Talin1, localizes to costanieres and intercalated disks, which are stable adhesions required for the assembly of mature striated muscle. Our results suggest that Talin1 is the primary link between integrins and actin in dynamic focal adhesions in undifferentiated, motile cells, but that Talin2 may serve as the link between integrins and the sarcomeric cytoskeleton in stable adhesion complexes in mature striated muscle.
引用
收藏
页码:157 / 173
页数:17
相关论文
共 77 条
[1]   Muscle differentiation: more complexity to the network of myogenic regulators [J].
Arnold, HH ;
Winter, B .
CURRENT OPINION IN GENETICS & DEVELOPMENT, 1998, 8 (05) :539-544
[2]   Talin contains three similar vinculin-binding sites predicted to form an amphipathic helix [J].
Bass, MD ;
Smith, BJ ;
Prigent, SA ;
Critchley, DR .
BIOCHEMICAL JOURNAL, 1999, 341 :257-263
[3]   LOCALIZATION OF TALIN IN SKELETAL AND CARDIAC MUSCLES [J].
BELKIN, AM ;
ZHIDKOVA, NI ;
KOTELIANSKY, VE .
FEBS LETTERS, 1986, 200 (01) :32-36
[4]   beta 1D integrin displaces the beta 1A isoform in striated muscles: Localization at junctional structures and signaling potential in nonmuscle cells [J].
Belkin, AM ;
Zhidkova, NI ;
Balzac, F ;
Altruda, F ;
Tomatis, D ;
Maier, A ;
Tarone, G ;
Koteliansky, VE ;
Burridge, K .
JOURNAL OF CELL BIOLOGY, 1996, 132 (1-2) :211-226
[5]   Pbx marks genes for activation by MyoD indicating a role for a homeodomain protein in establishing myogenic potential [J].
Berkes, CA ;
Bergstrom, DA ;
Penn, BH ;
Seaver, KJ ;
Knoepfler, PS ;
Tapscott, SJ .
MOLECULAR CELL, 2004, 14 (04) :465-477
[6]   Costameres: Repeating structures at the sarcolemma of skeletal muscle [J].
Bloch, RJ ;
Capetanaki, Y ;
O'Neill, A ;
Reed, P ;
Williams, MW ;
Resneck, WG ;
Porter, NC ;
Ursitti, JA .
CLINICAL ORTHOPAEDICS AND RELATED RESEARCH, 2002, (403) :S203-S210
[7]   Layilin, a novel talin-binding transmembrane protein homologous with C-type lectins, is localized in membrane ruffles [J].
Borowsky, ML ;
Hynes, RO .
JOURNAL OF CELL BIOLOGY, 1998, 143 (02) :429-442
[8]  
Brzóska E, 2004, CELL MOL BIOL LETT, V9, P723
[9]   TALIN - A CYTOSKELETAL COMPONENT CONCENTRATED IN ADHESION PLAQUES AND OTHER SITES OF ACTIN-MEMBRANE INTERACTION [J].
BURRIDGE, K ;
CONNELL, L .
CELL MOTILITY AND THE CYTOSKELETON, 1983, 3 (5-6) :405-417
[10]   A NEW PROTEIN OF ADHESION PLAQUES AND RUFFLING MEMBRANES [J].
BURRIDGE, K ;
CONNELL, L .
JOURNAL OF CELL BIOLOGY, 1983, 97 (02) :359-367