In vitro evidence for gut hormone stimulation release and dipeptidyl-peptidase IV inhibitory activity of protein hydrolysate obtained from cuttlefish (Sepia officinalis) viscera

被引：26

作者：

Cudennec, Benoit ^{[1
]}

Balti, Rafik ^{[2
]}

Ravallec, Rozenn ^{[1
]}

Caron, Juliette ^{[1
]}

Bougatef, Ali ^{[2
]}

Dhulster, Pascal ^{[1
]}

Nedjar, Naima ^{[1
]}

机构：

[1] Univ Lille, USC1281, EA7394, Charles Viollette Inst, Villeneuve Dascq, France

[2] Univ Sfax, Natl Sch Engn, Enzymes & Bioconvers, Sfax 3038, Tunisia

来源：

FOOD RESEARCH INTERNATIONAL | 2015年 / 78卷

关键词：

Bioactive peptides; DPP-IV inhibitors; Incretin effect; GLP-1; CCK; Diabetes; GLUCAGON-LIKE PEPTIDE-1; FUNCTIONAL-PROPERTIES; HEPATOPANCREAS; PURIFICATION; MECHANISMS; DIGESTION; FISH;

D O I：

10.1016/j.foodres.2015.10.003

中图分类号：

TS2 [食品工业];

学科分类号：

100403 [营养与食品卫生学];

摘要：

Two cuttlefish (Sepia officinalis) viscera protein hydrolysates were obtained with different enzymes extracted from cuttlefish and smooth hound (Mustellus mustellus). Their ability to stimulate the secretion of cholecystokinin (CCK) and glucagon-like peptide 1 (GLP-1), using the enteroendocrine STC-1 cell line, and to inhibit the DPP-IV activity during a simulated gastrointestinal digestion was assayed. The physico-chemical parameters of hydrolysates and their effects on intestinal cell viability were also determined. The hydrolysate obtained with cuttlefish ehzymes (CVPH1) appeared to be the most promising for all assessed bioactivities. Thus CVPH1 was able to stimulate CCK and active GLP-1 releasing activities of enteroendocrine cells without any cytotoxicity and to inhibit DPP-IV activity. Moreover, these actions were enhanced after gastrointestinal digestion and CVPH1 was also able to inhibit the intestinal DPP-IV activity of Caco-2 cells. These very promising findings highlight, via two different mechanisms, the positive effect of CVPH1 on GLP-1 actions. (C) 2015 Elsevier Ltd. All rights reserved.

引用

页码：238 / 245

页数：8

共 42 条

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[2]

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[3]

A heat-stable trypsin from the hepatopancreas of the cuttlefish (Sepia officinalis): Purification and characterisation [J].