Defeating Bacterial Resistance and Preventing Mammalian Cells Toxicity Through Rational Design of Antibiotic-Functionalized Nanoparticles

被引：62

作者：

Affonso de Oliveira, Jessica Fernanda ^{[1
,2
]}

Saito, Angela ^{[3
,4
]}

Bido, Ariadne Tuckmantel ^{[1
,2
]}

Kobarg, Joerg ^{[4
,5
]}

Stassen, Hubert Karl ^{[6
]}

Cardoso, Mateus Borba ^{[1
,2
]}

机构：

[1] CNPEM, Lab Nacl Nanotecnol LNNano, LNLS, Caixa Postal 6192, BR-13083970 Campinas, SP, Brazil

[2] Univ Estadual Campinas UNICAMP, Inst Quim, Caixa Postal 6154, BR-13083970 Campinas, SP, Brazil

[3] CNPEM, Lab Nacl Biociencias LNBio, Caixa Postal 6192, BR-13083970 Campinas, SP, Brazil

[4] Univ Estadual Campinas UNICAMP, Inst Biol, Dept Bioquim, Programa Posgrad Biol Func & Mol, Caixa Postal 6109, BR-13083970 Campinas, SP, Brazil

[5] Univ Estadual Campinas UNICAMP, Fac Ciencias Farmaceut, Caixa Postal 6029, BR-13083871 Campinas, SP, Brazil

[6] Univ Fed Rio Grande do Sul, Inst Quim, Caixa Postal 15003, BR-91501970 Porto Alegre, RS, Brazil

来源：

SCIENTIFIC REPORTS | 2017年 / 7卷

基金：

巴西圣保罗研究基金会;

关键词：

SILVER NANOPARTICLES; FORCE-FIELD; VALIDATION; STABILITY; SIZE;

D O I：

10.1038/s41598-017-01209-1

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

The rational synthesis of alternative materials is highly demanding due to the outbreak of infectious diseases and resistance to antibiotics. Herein, we report a tailored nanoantibiotic synthesis protocol where the antibiotic binding was optimized on the silver-silica core-shell nanoparticles surface to maximize biological responses. The obtained silver nanoparticles coated with mesoporous silica functionalized with ampicillin presented remarkable antimicrobial effects against susceptible and antibiotic-resistant Escherichia coli. In addition, these structures were not cell-death inducers and different steps of the mitotic cell cycle (prophase, anaphase and metaphase) were clearly identified. The superior biological results were attributed to a proper and tailored synthesis strategy.

引用

页数：10

共 37 条

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Ansari M. A., 2011, Biology and Medicine, V3, P141

[2]

Cell-death assessment by fluorescent and nonfluorescent cytosolic and nuclear staining techniques [J].