CD133 Identifies a Human Bone Marrow Stem/Progenitor Cell Sub-population With a Repertoire of Secreted Factors That Protect Against Stroke

被引：75

作者：

Bakondi, Benjamin ^{[1
]}

Shimada, Issei S. ^{[1
]}

Perry, Anthony ^{[2
]}

Munoz, James R. ^{[2
]}

Ylostalo, Joni ^{[2
]}

Howard, Alan B. ^{[3
,4
]}

Gregory, Carl A. ^{[2
]}

Spees, Jeffrey L. ^{[1
]}

机构：

[1] Univ Vermont, Dept Med, Colchester, VT 05446 USA

[2] Tulane Univ, Dept Med, Ctr Gene Therapy, Hlth Sci Ctr, New Orleans, LA 70118 USA

[3] Univ Vermont, Dept Math & Stat, Burlington, VT 05405 USA

[4] Univ Vermont, Dept Acad Comp, Burlington, VT USA

来源：

MOLECULAR THERAPY | 2009年 / 17卷 / 11期

基金：

美国国家卫生研究院;

关键词：

MESENCHYMAL STEM-CELLS; FACTOR RECEPTOR ANTIBODIES; STROMAL CELLS; HEMATOPOIETIC-STEM; PROGENITOR CELLS; GROWTH; MICROENVIRONMENT; EXPRESSION; TISSUES; NICHES;

D O I：

10.1038/mt.2009.185

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The reparative properties of bone marrow stromal cells (BMSCs) have been attributed in part to the paracrine action of secreted factors. We isolated typical human BMSCs by plastic adherence and compared them with BMSC sub-populations isolated by magnetic-activated cell sorting against CD133 (CD133-derived BMSCs, CD133BMSCs) or CD271 [p75 low-affinity nerve growth factor receptor (p75LNGFR), p75BMSCs]. Microarray assays of expressed genes, and enzyme-linked immunosorbent assays (ELISAs) of selected growth factors and cytokines secreted under normoxic and hypoxic conditions demonstrated that the three transit-amplifying progenitor cell populations were distinct from one another. CD133BMSC-conditioned medium (CdM) was superior to p75BMSC CdM in protecting neural progenitor cells against cell death during growth factor/nutrient withdrawal. Intracardiac (arterial) administration of concentrated CD133BMSC CdM provided neuroprotection and significantly reduced cortical infarct volumes in mice following cerebral ischemia. In support of the paracrine hypothesis for BMSC action, intra-arterial infusion of CD133BMSC CdM provided significantly greater protection against stroke compared with the effects of CD133BMSC (cell) administration. CdM from CD133BMSCs also provided superior protection against stroke compared with that conferred by CdM from p75BMSCs or typically isolated BMSCs. CD133 identifies a sub- population of nonhematopoietic stem/progenitor cells from adult human bone marrow, and CD133BMSC CdM may provide neuroprotection for patients with stroke.

引用

页码：1938 / 1947

页数：10

共 49 条

[1] A clonogenic common myeloid progenitor that gives rise to all myeloid lineages [J].

Akashi, K ;

Traver, D ;

Miyamoto, T ;

Weissman, IL .

NATURE, 2000, 404 (6774) :193-197

[2] Chemokines in hematopoiesis [J].

Broxmeyer, Hal E. .

CURRENT OPINION IN HEMATOLOGY, 2008, 15 (01) :49-58

[3] Novel markers for the prospective isolation of human MSC [J].

Buehring, Hans-Joerg ;

Battula, Venkata Lokesh ;

Treml, Sabrina ;

Schewe, Bernhard ;

Kanz, Lothar ;

Vogel, Wichard .

HEMATOPOIETIC STEM CELLS VI, 2007, 1106 :262-271

[4] Suppression of hepatocyte growth factor production impairs the ability of adipose-derived stem cells to promote ischemic tissue revascularization [J].

Cai, Liying ;

Johnstone, Brian H. ;

Cook, Todd G. ;

Liang, Zhong ;

Traktuev, Dmitry ;

Cornetta, Kenneth ;

Ingram, David A. ;

Rosen, Elliot D. ;

Marcha, Keith L. .

STEM CELLS, 2007, 25 (12) :3234-3243

[5] Osteoblastic cells regulate the haematopoietic stem cell niche [J].

Calvi, LM ;

Adams, GB ;

Weibrecht, KW ;

Weber, JM ;

Olson, DP ;

Knight, MC ;

Martin, RP ;

Schipani, E ;

Divieti, P ;

Bringhurst, FR ;

Milner, LA ;

Kronenberg, HM ;

Scadden, DT .

NATURE, 2003, 425 (6960) :841-846

[6]

CATTORETTI G, 1993, BLOOD, V81, P1726

[7] Therapeutic benefit of intravenous administration of bone marrow stromal cells after cerebral ischemia in rats [J].

Chen, JL ;

Li, Y ;

Wang, L ;

Zhang, ZG ;

Lu, DY ;

Lu, M ;

Chopp, M .

STROKE, 2001, 32 (04) :1005-1011

[8] Specific plasma membrane protein phenotype of culture-amplified and native human bone marrow mesenchymal stem cells [J].

Delorme, Bruno ;

Ringe, Jochen ;

Gallay, Nathalie ;

Le Vern, Yves ;

Kerboeuf, Dominique ;

Jorgensen, Christian ;

Rosset, Philippe ;

Sensebe, Luc ;

Layrolle, Pierre ;

Haeupl, Thomas ;

Charbord, Pierre .

BLOOD, 2008, 111 (05) :2631-2635

[9]

Dexter T M, 1984, Prog Clin Biol Res, V148, P13

[10] Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement [J].

Dominici, M. ;

Le Blanc, K. ;

Mueller, I. ;

Slaper-Cortenbach, I. ;

Marini, F. C. ;

Krause, D. S. ;

Deans, R. J. ;

Keating, A. ;

Prockop, D. J. ;

Horwitz, E. M. .

CYTOTHERAPY, 2006, 8 (04) :315-317

← 1 2 3 4 5 →