Visualizing three-dimensional root networks using computed tomography

被引:82
作者
Kaestner, Anders [1 ]
Schneebeli, Martin [1 ]
Graf, Frank [1 ]
机构
[1] ETH, Inst Terr Ecol, CH-8006 Zurich, Switzerland
关键词
soil; roots; computed tomography; image analysis; 3D; IMAGE-ANALYSIS; PLANT-ROOT; SOIL; RECONSTRUCTION; QUANTIFICATION; STABILITY; SYSTEMS; SIZE;
D O I
10.1016/j.geoderma.2006.04.009
中图分类号
S15 [土壤学];
学科分类号
0903 ; 090301 ;
摘要
We describe a method to reconstruct the root network from a three-dimensional image generated with computed tomography. The X-ray absorption of roots and the interface of the air-soil material is very similar and the contrast extremely low. The method consists of three steps: contrast enhancement using a non-linear diffusion filter, thresholding based on Rosin's method and extraction of the main features using a morphological connectivity algorithm. With this approach, we are also capable of reconstructing fine roots with high fidelity, which is shown with a performance experiment using artificial images with increasing noise. The method is applied to an X-ray microtomography data set of two alder roots, grown for 4months in a natural moraine soil. To simplify the soil material, the bare roots were transferred into a Plexiglas receptacle filled with quartz sand. The reconstructed image is compared with a photograph of the real roots. (c) 2006 Elsevier B.V. All rights reserved.
引用
收藏
页码:459 / 469
页数:11
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