The USA-derived transcriptional coactivator PC2 is a submodule of TRAP/SMCC and acts synergistically with other PCs

被引：110

作者：

Malik, S

Gu, W

Wu, WZ

Qin, J

Roeder, RG

机构：

[1] Rockefeller Univ, Biochem & Mol Biol Lab, New York, NY 10021 USA

[2] Baylor Coll Med, Dept Biochem, Houston, TX 77030 USA

[3] Baylor Coll Med, Dept Cell Biol, Houston, TX 77030 USA

来源：

MOLECULAR CELL | 2000年 / 5卷 / 04期

关键词：

D O I：

10.1016/S1097-2765(00)80254-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

PC2, the high-molecular weight constituent of the potent USA transcriptional coactivator fraction, was identified as a Mediator-like complex. Its composition resembles that of the TRAP/SMCC complex, but PC2 is distinguished by the prominent absence of the SRB10 and SRB11 kinase/cyclin pair, as well as several additional polypeptides. Furthermore, affinity-purified PC2, which lacks independent activity, acts in synergy with USA-derived coactivators PC3/topoisomerase I and PC4 to mediate the effects of a variety of activators (including VP16, the synthetic activator GAL4-AH, and the orphan nuclear receptor HNF4) and thus recapitulates partial USA coactivator function.

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收藏

页码：753 / 760

页数：8

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