Evaluation of mannitol salt agar, CHROMagar Staph aureus and CHROMagar MRSA for detection of meticillin-resistant Staphylococcus aureus from nasal swab specimens

被引:55
作者
Han, Zhuolin
Lautenbach, Ebbing
Fishman, Neil
Nachamkin, Irving [1 ]
机构
[1] Univ Penn, Sch Med, Dept Pathol & Lab Med, Philadelphia, PA 19104 USA
[2] Univ Penn, Sch Med, Dept Hosp Epidemiol & Infect Control, Philadelphia, PA 19104 USA
[3] Univ Penn, Sch Med, Dept Med, Div Infect Dis, Philadelphia, PA 19104 USA
[4] Univ Penn, Sch Med, Ctr Clin Epidemiol & Biostat, Dept Biostat & Epidemiol, Philadelphia, PA 19104 USA
关键词
D O I
10.1099/jmm.0.46777-0
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Mannitol salt agar (MSA), CHROMagar Staph aureus (CSA) and CHROMagar MRSA (CSAMRSA) were evaluated with nasal surveillance specimens for their ability to detect Staphylococcus aureus and meticillin-resistant S. aureus (MRSA). CSA was found to be more sensitive than MSA in detecting S. aureus (98 versus 84.3 %; P= 0.03). CSA and C:SA-MRSA were equivalent in the ability to detect MRSA at 24 h (89.7 versus 87.2 %) and at 48 h (94.9 versus 94.9 %). When combined with Staphaurex slide confirmation testing, both CSA and CSA-MRSA were highly specific (100 %) media for detecting MRSA from nasal swab specimens.
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收藏
页码:43 / 46
页数:4
相关论文
共 13 条
[1]  
Clinical and Laboratory Standards, 2005, PERF STAND ANT SUSC
[2]   Performance of CHROMagar MRSA medium for detection of methicillin-resistant Staphylococcus aureus [J].
Diederen, B ;
van Duijn, I ;
van Belkum, A ;
Willemse, P ;
van Keulen, P ;
Kluytmans, J .
JOURNAL OF CLINICAL MICROBIOLOGY, 2005, 43 (04) :1925-1927
[3]   Multicenter evaluation of BBL CHROMagar MRSA medium for direct detection of methicillin-resistant Staphylococcus aureus from surveillance cultures of the anterior nares [J].
Flayhart, D ;
Hindler, JF ;
Bruckner, DA ;
Hall, G ;
Shrestha, RK ;
Vogel, SA ;
Richter, SS ;
Howard, W ;
Walther, R ;
Carroll, KC .
JOURNAL OF CLINICAL MICROBIOLOGY, 2005, 43 (11) :5536-5540
[4]   Rapid detection of methicillin-resistant Staphylococcus aureus directly from sterile or nonsterile clinical samples by a new molecular assay [J].
Francois, P ;
Pittet, D ;
Bento, M ;
Pepey, B ;
Vaudaux, P ;
Lew, D ;
Schrenzel, J .
JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (01) :254-260
[5]   Evaluation of CHROMagar Staph, aureus, a new chromogenic medium, for isolation and presumptive identification of Staphylococcus aureus from human clinical specimens [J].
Gaillot, O ;
Wetsch, M ;
Fortineau, N ;
Berche, P .
JOURNAL OF CLINICAL MICROBIOLOGY, 2000, 38 (04) :1587-1591
[6]   SHEA guideline for preventing nosocomial transmission of multidrug-resistant strains of staphylococcus aureus and enterococcus [J].
Muto, CA ;
Jernigan, JA ;
Ostrowsky, BE ;
Richet, HM ;
Jarvis, WR ;
Boyce, JM ;
Farr, BM .
INFECTION CONTROL AND HOSPITAL EPIDEMIOLOGY, 2003, 24 (05) :362-386
[7]   Development and evaluation of a chromogenic agar medium for methicillin-resistant Staphylococcus aureus [J].
Perry, JD ;
Davies, A ;
Butterworth, LA ;
Hopley, ALJ ;
Nicholson, A ;
Gould, FK .
JOURNAL OF CLINICAL MICROBIOLOGY, 2004, 42 (10) :4519-4523
[8]   Evaluation of S-aureus ID, a new chromogenic agar medium for detection of Staphylococcus aureus [J].
Perry, JD ;
Rennison, C ;
Butterworth, LA ;
Hopley, ALJ ;
Gould, FK .
JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (12) :5695-5698
[9]   The economic impact of Staphylococcus aureus infection in New York City hospitals [J].
Rubin, RJ ;
Harrington, CA ;
Poon, A ;
Dietrich, K ;
Greene, JA ;
Moiduddin, A .
EMERGING INFECTIOUS DISEASES, 1999, 5 (01) :9-17
[10]   Prospective comparison of a new chromogenic medium, MRSASelect, to CHROMagar MRSA and mannitol-salt medium supplemented with oxacillin or cefoxitin for detection of methicillin-resistant Staphylococcus aureus [J].
Stoakes, L ;
Reyes, R ;
Daniel, J ;
Lennox, G ;
John, MA ;
Lannigan, R ;
Hussain, Z .
JOURNAL OF CLINICAL MICROBIOLOGY, 2006, 44 (02) :637-639