The role of RNA editing by ADARs in RNAi

被引:104
作者
Knight, SW
Bass, BL
机构
[1] Univ Utah, Dept Biochem, Salt Lake City, UT 84132 USA
[2] Univ Utah, Howard Hughes Med Inst, Salt Lake City, UT 84132 USA
关键词
D O I
10.1016/S1097-2765(02)00649-4
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Adenosine deaminases that act on RNA (ADARs) are RNA-editing enzymes that deaminate adenosines to create inosines in double-stranded RNA (dsRNA). Here we demonstrate that ADARs are not required for RNA interference (RNAi) and that they do not antagonize the pathway to a detectable level when RNAi is initiated by injecting dsRNA. We find, however, that transgenes expressed in the somatic tissues of wild-type animals are silenced in strains with deletions in the two genes encoding ADARs, adr-1 and adr-2. Transgene-induced gene silencing in adr-1;adr-2 mutants depends on genes required for RNAi, suggesting that a dsRNA intermediate is involved. In wild-type animals we detect edited dsRNA corresponding to transgenes, and we propose that editing of this dsRNA prevents somatic transgenes from initiating RNAi in wild-type animals.
引用
收藏
页码:809 / 817
页数:9
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