Nucleotides and two functional states of hsp90

被引:220
作者
Sullivan, W
Stensgard, B
Caucutt, G
Bartha, B
McMahon, N
Alnemri, ES
Litwack, G
Toft, D
机构
[1] MAYO CLIN & MAYO GRAD SCH MED,DEPT BIOCHEM & MOL BIOL,ROCHESTER,MN 55905
[2] THOMAS JEFFERSON UNIV,JEFFERSON MED COLL,DEPT BIOCHEM & MOL PHARMACOL,PHILADELPHIA,PA 19107
关键词
D O I
10.1074/jbc.272.12.8007
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Previous studies have demonstrated the ATP-dependent formation of a complex containing the heat shock protein hsp90, the unique hsp90 binding protein p23, and one of three high molecular weight immunophilins. In the present study, hsp90 and p23 are shown 60 form a complex that requires elevated temperature and ATP/Mg2+. Complex formation is strongly promoted by molybdate and by the nonionic detergent Nonidet P-40. ADP and the benzoquinone ansamycin, geldanamycin, are potent inhibitors of complex formation. The ATP-dependent process alters the state of hsp90, not p23, and influences the ability of hsp90 to bind to phenyl-Sepharose. Conversion of hsp90 to the ATP-bound state lowers its affinity for phenyl-Sepharose. These results show that hsp90 can exist in at least two functional states, one able to bind p23 and the other with a high affinity for hydrophobic resins. A model is presented where these states are dictated by the binding of either ATP or ADP.
引用
收藏
页码:8007 / 8012
页数:6
相关论文
共 46 条
  • [1] THE STEROID-BINDING DOMAIN INFLUENCES INTRACELLULAR SOLUBILITY OF THE BACULOVIRUS OVEREXPRESSED GLUCOCORTICOID AND MINERALOCORTICOID RECEPTORS
    ALNEMRI, ES
    LITWACK, G
    [J]. BIOCHEMISTRY, 1993, 32 (20) : 5387 - 5393
  • [2] BLAGOSKLONNY MV, 1995, ONCOGENE, V11, P933
  • [3] HSP82 IS AN ESSENTIAL PROTEIN THAT IS REQUIRED IN HIGHER CONCENTRATIONS FOR GROWTH OF CELLS AT HIGHER TEMPERATURES
    BORKOVICH, KA
    FARRELLY, FW
    FINKELSTEIN, DB
    TAULIEN, J
    LINDQUIST, S
    [J]. MOLECULAR AND CELLULAR BIOLOGY, 1989, 9 (09) : 3919 - 3930
  • [4] Chaperone function of Hsp90-associated proteins
    Bose, S
    Weikl, T
    Bugl, H
    Buchner, J
    [J]. SCIENCE, 1996, 274 (5293) : 1715 - 1717
  • [5] ALUMINOFLUORIDE AND BERYLLOFLUORIDE COMPLEXES - NEW PHOSPHATE ANALOGS IN ENZYMOLOGY
    CHABRE, M
    [J]. TRENDS IN BIOCHEMICAL SCIENCES, 1990, 15 (01) : 6 - 10
  • [6] Interactions of p60, a mediator of progesterone receptor assembly, with heat shock proteins hsp90 and hsp70
    Chen, SY
    Prapapanich, V
    Rimerman, RA
    Honore, B
    Smith, DF
    [J]. MOLECULAR ENDOCRINOLOGY, 1996, 10 (06) : 682 - 693
  • [7] CSERMELY P, 1991, J BIOL CHEM, V266, P4943
  • [8] CSERMELY P, 1993, J BIOL CHEM, V268, P1901
  • [9] CZAR MJ, 1994, J BIOL CHEM, V269, P11155
  • [10] Molecular chaperone machines: Chaperone activities of the cyclophilin Cyp-40 and the steroid aporeceptor-associated protein p23
    Freeman, BC
    Toft, DO
    Morimoto, RI
    [J]. SCIENCE, 1996, 274 (5293) : 1718 - 1720