The rpoD1 gene of Synechococcus sp strain PCC 7942 encodes the principal sigma factor of RNA polymerase

被引：6

作者：

Sugimoto, Y ^{[1
]}

Tanaka, K ^{[1
]}

Masuda, S ^{[1
]}

Takahashi, H ^{[1
]}

机构：

[1] UNIV TOKYO, INST MOL & CELLULAR BIOSCI, BUNKYO KU, TOKYO 113, JAPAN

来源：

JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY | 1997年 / 43卷 / 01期

关键词：

amino acid sequence; cyanobacterium; principal sigma factor; purification; RNA polymerase; rpoD1; gene; Synechococcus sp;

D O I：

10.2323/jgam.43.17

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

RNA polymerase was purified from the unicellular cyanobacterium, Synechococcus sp. strain PCC 7942, and found to be associated with a 52 kilodalton (kDa) polypeptide. The determined N-terminal sequence of the polypeptide was identical to the predicted amino-acid sequence of the rpoD1 gene product. Furthermore, the rpoD1 gene is suggested to be indispensable for viability by the inability to disrupt the gene. These results indicate that the rpoD1 gene product is the principal sigma factor of RNA polymerase.

引用

页码：17 / 21

页数：5

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