Comparison of the spatial homogeneity of physico-chemical parameters and bacterial 16S rRNA genes in sediment samples from a dumping site for dredging sludge

被引:40
作者
Boon, N [1 ]
Marlé, C [1 ]
Top, EM [1 ]
Verstraete, W [1 ]
机构
[1] State Univ Ghent, Fac Agr & Appl Biol Sci, Lab Microbial Ecol & Technol, B-9000 Ghent, Belgium
关键词
D O I
10.1007/s002530000325
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The homogeneity of the microbial community structure of a sediment landfill was examined by a culture-independent method and compared with physico-chemical parameters, i.e. organic matter, CaCO3 content, pH, and texture. Total genomic DNA was extracted from samples derived from different places and depths. After amplification with two different primer sets of partial bacterial 16S rRNA genes, the products were separated by denaturing gradient gel electrophoresis (DGGE). The DGGE fingerprints of different sediment samples taken in regular patterns at the same depth were similar, which indicates a spatial homogeneity in the numerically dominant bacterial populations in a landfill over 10,000 m(2) in size. In a vertical column of approx. 10 m, only some differences in a few bands of the bacterial community structure were observed between samples taken from different depths. This DNA homogeneity coincided with a similar homogeneity of the physico-chemical parameters in the landfill at this site. Nevertheless, the DGGE technique revealed small differences in less prominent bacteria and was capable of separating the upper and lower samples of one column into two clusters. It therefore seems more sensitive than the physico-chemical approach for characterising the homogeneity of an environmental habitat.
引用
收藏
页码:742 / 747
页数:6
相关论文
共 22 条
[1]   PHYLOGENETIC IDENTIFICATION AND IN-SITU DETECTION OF INDIVIDUAL MICROBIAL-CELLS WITHOUT CULTIVATION [J].
AMANN, RI ;
LUDWIG, W ;
SCHLEIFER, KH .
MICROBIOLOGICAL REVIEWS, 1995, 59 (01) :143-169
[2]  
ATLAS RM, 1984, ADV MICROB ECOL, V7, P1
[3]   Hydraulic conductivity of compacted soil treated with biofilm [J].
Dennis, ML ;
Turner, JP .
JOURNAL OF GEOTECHNICAL AND GEOENVIRONMENTAL ENGINEERING, 1998, 124 (02) :120-127
[4]  
Donner G., 1996, ARCH HYDROBIOL SPEC, V48, P7
[5]  
Dunbar J, 1999, APPL ENVIRON MICROB, V65, P1662
[6]  
Eichner CA, 1999, APPL ENVIRON MICROB, V65, P102
[7]  
El Fantroussi S, 1999, APPL ENVIRON MICROB, V65, P982
[8]   Introduction and PCR detection of Desulfomonile tiedjei in soil slurry microcosms [J].
El Fantroussi S. ;
Mahillon J. ;
Naveau H. ;
Agathos S.N. .
Biodegradation, 1997, 8 (2) :125-133
[9]   EFFECT OF GENOME SIZE AND RRN GENE COPY NUMBER ON PCR AMPLIFICATION OF 16S RIBOSOMAL-RNA GENES FROM A MIXTURE OF BACTERIAL SPECIES [J].
FARRELLY, V ;
RAINEY, FA ;
STACKEBRANDT, E .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1995, 61 (07) :2798-2801
[10]   Spatial homogeneity of abundant bacterial 16S rRNA molecules in grassland soils [J].
Felske, A ;
Akkermans, ADL .
MICROBIAL ECOLOGY, 1998, 36 (01) :31-36