Development of the single-cell MALDI-TOF (matrix-assisted laser desorption/ionization time-of-flight) mass-spectroscopic assay

被引：17

作者：

Shimizu, M ^{[1
]}

Ojima, N ^{[1
]}

Ohnishi, H ^{[1
]}

Shingaki, T ^{[1
]}

Hirakawa, Y ^{[1
]}

Masujima, T ^{[1
]}

机构：

[1] Hiroshima Univ, Sch Med, Inst Pharmaceut Sci, Dept Analyt Life Sci,Minami Ku, Hiroshima 7348551, Japan

来源：

ANALYTICAL SCIENCES | 2003年 / 19卷 / 01期

关键词：

D O I：

10.2116/analsci.19.49

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

MALDI-TOF mass spectrometry was used to detect intracellular molecules from a single intact cell on monolayers of other cells. Intracellular molecules, e.g., histamine, were gradually increased in a mouse bone marrow-derived mast cell by a maturation process. A single cell was captured by a microsuction pipette, and the mass spectra of intracellular histamine were measured directly. Finally, the time course of the intracellular molecular contents and the maturation stage from a single cell were estimated.

引用

页码：49 / 53

页数：5

共 19 条

[1] Spontaneous polyploidization results in apoptosis in a Meth-A tumor cell line [J].