Effect of metabolic acidosis on the potassium content of bone

被引:38
作者
Bushinsky, DA
Gavrilov, K
Chabala, JM
Featherstone, JDB
LeviSetti, R
机构
[1] STRONG MEM HOSP,NEPHROL UNIT,ROCHESTER,NY 14642
[2] UNIV CHICAGO,DEPT PHYS,ENRICO FERMI INST,CHICAGO,IL 60637
[3] UNIV CALIF SAN FRANCISCO,DEPT RESTORAT DENT,SAN FRANCISCO,CA 94143
关键词
D O I
10.1359/jbmr.1997.12.10.1664
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Metabolic acidosis induces resorption of cultured bone, resulting in a net efflux of calcium (Ca) from the bone and an apparent loss of mineral potassium (K), However, in these organ cultures, there is diffusion of K between the medium and the crystal lattice, causing difficulty in interpretation of the acid-induced changes in mineral ion composition, To determine the effects of acidosis on bone mineral K, we injected 4-day-old neonatal mice with pure stable isotope K-41, equal to similar to 5% of their total body K. Calvariae were dissected 24 h later and then cultured for 24 h in medium without added K-41, either at pH similar to 7.4 (Ctl) or at pH similar to 7.1 (Ac), with or without the osteoclastic inhibitor calcitonin (3 x 10(-9) M, CT), The bone isotopic ion content was determined with a high-resolution scanning ion microprobe utilizing secondary ion mass spectrometry, K-41 is present in nature at 6.7% of total K, The injected K-41 raised the ratio of bone K-41/(K-39 + K-41) to 9.8 +/- 0.5% On the surface (ratios of counts per second of detected secondary ions, mean +/- 95% confidence interval) but did not alter the ratio in the interior (6.9 +/- 0.4%), indicating biological incorporation of the K-41 into the mineral surface, The ratios of K-41/Ca-40 on the surface of Ctl calvariae was 14.4 +/- 1.2, indicating that bone mineral surface is rich in K compared with Ca, Compared with Ctl, Ac caused a marked increase in the net Ca efflux from bone that was blocked by CT, Ac also induced a marked fall in the ratio of K-41/Ca-40 on the surface of the calvariae (4.3 +/- 0.5, p < 0.01 VS, Ctl), which was partially blocked by CT (8.2 +/- 0.9, p < 0.01 vs, Ctl and vs, Ac), indicating that Ac causes a greater release of bone mineral K than Ca which is partially blocked by CT, Thus, bone mineral surface is rich in K relative to Ca, acidosis induces a greater release of surface mineral K than Ca, and osteoclastic function is necessary to support the enriched levels of surface mineral K in the presence of acidosis.
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页码:1664 / 1671
页数:8
相关论文
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