Maf1 is an essential mediator of diverse signals that repress RNA polymerase III transcription

被引：188

作者：

Upadhya, R ^{[1
]}

Lee, JH ^{[1
]}

Willis, IM ^{[1
]}

机构：

[1] Albert Einstein Coll Med, Dept Biochem, Bronx, NY 10461 USA

来源：

MOLECULAR CELL | 2002年 / 10卷 / 06期

关键词：

D O I：

10.1016/S1097-2765(02)00787-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Maf1 is a putative repressor of RNA polymerase (pol) III transcription that is conserved from yeast to humans. Here we show that Maf1 is a common component of multiple signaling pathways in S. cerevesiae that sense changes in the cellular environment and repress pol III transcription. Signaling pathways activated in response to rapamycin-induced nutrient limitation, DNA damage, and secretory pathway defects all require Maf1 in order to affect pol III transcriptional repression. In addition, Maf1 was required for repression of pol III transcription during the normal yeast growth cycle. Biochemical studies identified the initiation factor TFIIIB as a target of Maf1-dependent repression and revealed a defect in TFIIIB-DNA complex assembly under repressing conditions.

引用

页码：1489 / 1494

页数：6

共 34 条

[1] Overlapping functions of the pRb family in the regulation of rRNA synthesis [J].

Ciarmatori, S ;

Scott, PH ;

Sutcliffe, JE ;

McLees, A ;

Alzuherri, HM ;

Dannenberg, JH ;

te Riele, H ;

Grummt, I ;

Voit, R ;

White, RJ .

MOLECULAR AND CELLULAR BIOLOGY, 2001, 21 (17) :5806-5814

[2] Genomic expression programs in the response of yeast cells to environmental changes [J].

Gasch, AP ;

Spellman, PT ;

Kao, CM ;

Carmel-Harel, O ;

Eisen, MB ;

Storz, G ;

Botstein, D ;

Brown, PO .

MOLECULAR BIOLOGY OF THE CELL, 2000, 11 (12) :4241-4257

[3] Functional organization of the yeast proteome by systematic analysis of protein complexes [J].

Gavin, AC ;

Bösche, M ;

Krause, R ;

Grandi, P ;

Marzioch, M ;

Bauer, A ;

Schultz, J ;

Rick, JM ;

Michon, AM ;

Cruciat, CM ;

Remor, M ;

Höfert, C ;

Schelder, M ;

Brajenovic, M ;

Ruffner, H ;

Merino, A ;

Klein, K ;

Hudak, M ;

Dickson, D ;

Rudi, T ;

Gnau, V ;

Bauch, A ;

Bastuck, S ;

Huhse, B ;

Leutwein, C ;

Heurtier, MA ;

Copley, RR ;

Edelmann, A ;

Querfurth, E ;

Rybin, V ;

Drewes, G ;

Raida, M ;

Bouwmeester, T ;

Bork, P ;

Seraphin, B ;

Kuster, B ;

Neubauer, G ;

Superti-Furga, G .

NATURE, 2002, 415 (6868) :141-147

[4] The RNA polymerase III transcription apparatus [J].

Geiduschek, EP ;

Kassavetis, GA .

JOURNAL OF MOLECULAR BIOLOGY, 2001, 310 (01) :1-26

[5] TATA binding protein-associated CK2 transduces DNA damage, signals to the RNA polymerase III transcriptional machinery [J].

Ghavidel, A ;

Schultz, MC .

CELL, 2001, 106 (05) :575-584

[6] MAP kinase pathways in the yeast Saccharomyces cerevisiae [J].

Gustin, MC ;

Albertyn, J ;

Alexander, M ;

Davenport, K .

MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 1998, 62 (04) :1264-+

[7] Systematic identification of protein complexes in Saccharomyces cerevisiae by mass spectrometry [J].

Ho, Y ;

Gruhler, A ;

Heilbut, A ;

Bader, GD ;

Moore, L ;

Adams, SL ;

Millar, A ;

Taylor, P ;

Bennett, K ;

Boutilier, K ;

Yang, LY ;

Wolting, C ;

Donaldson, I ;

Schandorff, S ;

Shewnarane, J ;

Vo, M ;

Taggart, J ;

Goudreault, M ;

Muskat, B ;

Alfarano, C ;

Dewar, D ;

Lin, Z ;

Michalickova, K ;

Willems, AR ;

Sassi, H ;

Nielsen, PA ;

Rasmussen, KJ ;

Andersen, JR ;

Johansen, LE ;

Hansen, LH ;

Jespersen, H ;

Podtelejnikov, A ;

Nielsen, E ;

Crawford, J ;

Poulsen, V ;

Sorensen, BD ;

Matthiesen, J ;

Hendrickson, RC ;

Gleeson, F ;

Pawson, T ;

Moran, MF ;

Durocher, D ;

Mann, M ;

Hogue, CWV ;

Figeys, D ;

Tyers, M .

NATURE, 2002, 415 (6868) :180-183

[8] Comparison of the RNA polymerase III transcription machinery in Schizosaccharomyces pombe, Saccharomyces cerevisiae and human [J].

Huang, Y ;

Maraia, RJ .

NUCLEIC ACIDS RESEARCH, 2001, 29 (13) :2675-2690

[9] CK2 forms a stable complex with TFIII3 and activates RNA polymerase III transcription in human cells [J].

Johnston, IM ;

Allison, SJ ;

Morton, JP ;

Schramm, L ;

Scott, PH ;

White, RJ .

MOLECULAR AND CELLULAR BIOLOGY, 2002, 22 (11) :3757-3768

[10] RIBOSOME SYNTHESIS DURING THE GROWTH-CYCLE OF SACCHAROMYCES-CEREVISIAE [J].

JU, QD ;

WARNER, JR .

YEAST, 1994, 10 (02) :151-157

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