Functional interactions of DNA topoisomerases with a human replication origin

被引：44

作者：

Abdurashidova, Gulnara

Radulescu, Sorina

Sandoval, Oscar

Zahariev, Sotir

Danailov, Miltcho B.

Demidovich, Alexander

Santamaria, Laura

Biamonti, Giuseppe

Riva, Silvano

Falaschi, Arturo

机构：

[1] ICGEB, Int Ctr Genet Engn & Biotechnol, Mol Biol Grp, I-34012 Trieste, Italy

[2] Sincrotrone Trieste, I-34012 Trieste, Italy

[3] CNR, Ist Genet Mol, Mol Biol Sect, I-27100 Pavia, Italy

[4] Scuola Normale Super Pisa, Mol Biol Lab, Pisa, Italy

[5] CNR, Ist Fisiol Clin, Lab Gene & Mol Therapy, I-56100 Pisa, Italy

来源：

EMBO JOURNAL | 2007年 / 26卷 / 04期

关键词：

DNA replication regulation; DNA topology; lamin B2 origin;

D O I：

10.1038/sj.emboj.7601578

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The human DNA replication origin, located in the lamin B2 gene, interacts with the DNA topoisomerases I and II in a cell cycle-modulated manner. The topoisomerases interact in vivo and in vitro with precise bonds ahead of the start sites of bidirectional replication, within the pre-replicative complex region; topoisomerase I is bound in M, early G1 and G1/S border and topoisomerase II in M and the middle of G1. The Orc2 protein competes for the same sites of the origin bound by either topoisomerase in different moments of the cell cycle; furthermore, it interacts on the DNA with topoisomerase II during the assembly of the pre-replicative complex and with DNA-bound topoisomerase I at the G1/S border. Inhibition of topoisomerase I activity abolishes origin firing. Thus, the two topoisomerases are closely associated with the replicative complexes, and DNA topology plays an essential functional role in origin activation.

引用

页码：998 / 1009

页数：12

共 36 条

[1] Start sites of bidirectional DNA synthesis at the human lamin B2 origin [J].