Control of a multisubunit DNA motor by a thermoresponsive polymer switch

被引：50

作者：

Pennadam, SS

Lavigne, MD

Dutta, CF

Firman, K

Mernagh, D

Górecki, DC

Alexander, C

机构：

[1] Univ Portsmouth, Inst Biomed & Biomol Sci, Sch Pharm & Biomed Sci, Portsmouth PO1 2DT, Hants, England

[2] Univ Portsmouth, Inst Biomed & Biomol Sci, Sch Biol Sci, Portsmouth PO1 2DT, Hants, England

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2004年 / 126卷 / 41期

关键词：

D O I：

10.1021/ja045275j

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The conjugation of thermoresponsive polymers to multisubunit, multifunctional hybrid type 1 DNA restriction-modification (R-M) enzymes enables temperature-controlled switching" of DNA methylation by the conjugate. Polymers attached to the enzyme at a subunit distal to the methylation subunit allow retention of DNA recognition and ATPase activity while controlling methylation of plasmid DNA. This regulation of enzyme activity arises from the coil-globule phase transitions of the polymer as shown in light scattering and gel retardation assays. Copyright © 2004 American Chemical Society."

引用

页码：13208 / 13209

页数：2

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