Kinetics of myosin subfragment-1-induced condensation of G-actin into oligomers, precursors in the assembly of F-actin-S-1. Role of the tightly bound metal ion and ATP hydrolysis

In a low ionic strength buffer and in the absence of free ATP, the interaction of G-actin (G) with myosin subfragment-1 (S-1) leads to the formation of arrowhead-decorated F-actin-S-1 filaments, through a series of elementary steps. The initial formation of GS and G(2)S complexes is followed by their condensation into short oligomers, The kinetics of formation of G-actin-S-1 oligomers have been monitored in a stopped-flow apparatus using a combination of light scattering and fluorescence of NBD-labeled actin, Oligomers appear more stable and are formed at a faster rate from MgATP-G-actin than from CaATP-G-actin. The actin-bound ATP is hydrolyzed when oligomers are formed from MgATP-G-actin, not when they are formed from CaATP-G-actin. The formation of oligomers is energetically favored in the presence of cytochalasin D. All data are consistent with the view that the actin-actin interactions which take place upon condensation of GS and G(2)S into oligomers are very similar to lateral actin-actin interactions along the short pitch helix of actin filaments, which are involved in actin nucleation, These interactions trigger ATP hydrolysis on actin.