A novel Ser O-glucuronidation in acidic proline-rich proteins identified by tandem mass spectrometry

被引：12

作者：

Jonsson, AP

Griffiths, WJ

Bratt, P

Johansson, I

Strömberg, N

Jörnvall, H

Bergman, T ^{[1
]}

机构：

[1] Karolinska Inst, Dept Med Biochem & Biophys, SE-17177 Stockholm, Sweden

[2] Umea Univ, Dept Cariol, SE-90187 Umea, Sweden

来源：

FEBS LETTERS | 2000年 / 475卷 / 02期

关键词：

tandem mass spectrometry; electrospray; acidic proline-rich protein; post-translational modification; phosphorylation; glucuronidation;

D O I：

10.1016/S0014-5793(00)01645-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Human acidic proline-rich salivary protein PRP-1 and its C-terminally truncated form PRP-3 mere analyzed by electrospray tandem mass spectrometry, Post-translational modifications were detected and characterized. A pyroglutamic acid residue was demonstrated at the N-terminus, Ser-8 and Ser-22 were shown to be phosphorylated and an O-linked glucuronic acid conjugation mas identified. The latter modification was located to Ser-17 and found to be present in approximately 40% of the polypeptides, (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：131 / 134

页数：4

共 20 条

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