Cloning of two novel human importin-alpha subunits and analysis of the expression pattern of the importin-alpha protein family

被引：204

作者：

Kohler, M

Ansieau, S

Prehn, S

Leutz, A

Haller, H

Hartmann, E

机构：

[1] MAX DELBRUCK CTR MOL MED, D-13125 BERLIN, GERMANY

[2] HUMBOLDT UNIV BERLIN, FRANZ VOLHARD CLIN, BERLIN, GERMANY

[3] HUMBOLDT UNIV BERLIN, DEPT BIOCHEM CHARITE, BERLIN, GERMANY

来源：

FEBS LETTERS | 1997年 / 417卷 / 01期

关键词：

nuclear transport; importin-alpha; protein expression; nuclear signaling;

D O I：

10.1016/S0014-5793(97)01265-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The import of many proteins into the nucleus is mediated by the importin-alpha/beta heterodimer. While only one importin-beta gene has been found, several forms of importin-alpha have been described. In addition to the three human importin-alpha s already identified, we report here the primary structure of two new human importin-alpha proteins. The five known human importin-alpha subunits can be classified into three subfamilies that appear conserved in higher eukaryotic organisms. We show by immunoblotting that the different importin-alpha subfamilies are expressed in a variety of human tissues and mammalian cell lines. (C) 1997 Federation of European Biochemical Societies.

引用

页码：104 / 108

页数：5

共 31 条

[1] CYTOSOLIC PROTEINS THAT SPECIFICALLY BIND NUCLEAR LOCATION SIGNALS ARE RECEPTORS FOR NUCLEAR IMPORT
ADAM, SA
GERACE, L
[J]. CELL, 1991, 66 (05) : 837 - 847
[2] NUCLEAR-PROTEIN IMPORT IN PERMEABILIZED MAMMALIAN-CELLS REQUIRES SOLUBLE CYTOPLASMIC FACTORS
ADAM, SA
MARR, RS
GERACE, L
[J]. JOURNAL OF CELL BIOLOGY, 1990, 111 (03) : 807 - 816
[3] SEQUENCE AND CHARACTERIZATION OF CYTOPLASMIC NUCLEAR-PROTEIN IMPORT FACTOR P97
CHI, NC
ADAM, EJH
ADAM, SA
[J]. JOURNAL OF CELL BIOLOGY, 1995, 130 (02) : 265 - 274
[4] RAG-1 INTERACTS WITH THE REPEATED AMINO-ACID MOTIF OF THE HUMAN HOMOLOG OF THE YEAST PROTEIN SRP1
CORTES, P
YE, ZS
BALTIMORE, D
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (16) : 7633 - 7637
[5] RCH1, A PROTEIN THAT SPECIFICALLY INTERACTS WITH THE RAG-1 RECOMBINATION-ACTIVATING PROTEIN
CUOMO, CA
KIRCH, SA
GYURIS, J
BRENT, R
OETTINGER, MA
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (13) : 6156 - 6160
[6] A POLYPEPTIDE DOMAIN THAT SPECIFIES MIGRATION OF NUCLEOPLASMIN INTO THE NUCLEUS
DINGWALL, C
SHARNICK, SV
LASKEY, RA
[J]. CELL, 1982, 30 (02) : 449 - 458
[7] MOVEMENT OF A CARYOPHILIC PROTEIN THROUGH THE NUCLEAR-PORES OF OOCYTES
FELDHERR, CM
KALLENBACH, E
SCHULTZ, N
[J]. JOURNAL OF CELL BIOLOGY, 1984, 99 (06) : 2216 - 2222
[8] Nuclear protein import
Gorlich, D
[J]. CURRENT OPINION IN CELL BIOLOGY, 1997, 9 (03) : 412 - 419
[9] DISTINCT FUNCTIONS FOR THE 2 IMPORTIN SUBUNITS IN NUCLEAR-PROTEIN IMPORT
GORLICH, D
VOGEL, F
MILLS, AD
HARTMANN, E
LASKEY, RA
[J]. NATURE, 1995, 377 (6546) : 246 - 248
[10] A MAMMALIAN HOMOLOG OF SEC61P AND SECYP IS ASSOCIATED WITH RIBOSOMES AND NASCENT POLYPEPTIDES DURING TRANSLOCATION
GORLICH, D
PREHN, S
HARTMANN, E
KALIES, KU
RAPOPORT, TA
[J]. CELL, 1992, 71 (03) : 489 - 503

← 1 2 3 4 →