Crystallization and electron paramagnetic resonance characterization of the complex of photosystem I with its natural electron acceptor ferredoxin

被引:22
作者
Fromme, P
Bottin, H
Krauss, N
Sétif, P
机构
[1] Tech Univ Berlin, Fak 2, Inst Chem, Max Volmer Lab, D-10623 Berlin, Germany
[2] Arizona State Univ, Dept Chem & Biochem, Tempe, AZ 85287 USA
[3] CEA, Dept Biol Joliot Curie, Serv Bioenerget, F-91191 Gif Sur Yvette, France
[4] CNRS, URA 2096, F-91191 Gif Sur Yvette, France
[5] Free Univ Berlin, Inst Chem Kristallog, D-14195 Berlin, Germany
关键词
D O I
10.1016/S0006-3495(02)73942-8
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
The formation of a transient complex between photosystem I and ferredoxin is involved in the process of ferredoxin photoreduction in oxygenic photosynthetic organisms. Reduced ferredoxin is an essential redox intermediate involved in many assimilatory processes and is necessary for the reduction of NADP(+) to NADPH. Single crystals from a complex of photosystem I with ferredoxin were grown using PEG 400 and CaCl2 as precipitation agents. The crystals diffract x-rays to a resolution of 7-8 Angstrom. The space group was determined to be orthorhombic with the unit cell dimensions a = 194 Angstrom, b = 208 Angstrom, and c = 354 Angstrom. The crystals contain photosystem I and ferredoxin in a 1:1 ratio. Electron paramagnetic resonance (EPR) measurements on these crystals are reported, where EPR signals of the three [4Fe-4S] clusters F-A, F-B, F-X, and the [2Fe-2S] cluster of ferredoxin were detected. From the EPR spectra observed at three particular orientations of the crystal in the magnetic field, the full orientation pattern of the F-A(-) g-tensor was simulated. This simulation is consistent with the presence of 12 magnetically inequivalent F-A(-) clusters per unit cell with the C-3 axis of the PSI trimers oriented at (23degrees, 72degrees, 77degrees) to the unit cell axes.
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页码:1760 / 1773
页数:14
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