Recovery of Burkholderia cenocepacia strain PHDC from cystic fibrosis patients in Europe

被引:42
作者
Coenye, T
Spilker, T
Van Schoor, A
LiPuma, JJ
Vandamme, P
机构
[1] Univ Ghent, Microbiol Lab, B-9000 Ghent, Belgium
[2] Univ Michigan, Dept Pediat & Communicable Dis, Ann Arbor, MI 48109 USA
关键词
D O I
10.1136/thx.2003.019810
中图分类号
R56 [呼吸系及胸部疾病];
学科分类号
摘要
Background: Burkholderia cenocepacia can cause life threatening respiratory tract infections in patients with cystic fibrosis (CF) and has a significant impact on survival. There is extensive evidence for patient to patient spread and nosocomial transmission of this organism, and several widespread B cenocepacia strains have been described including the transatlantic ET12 clone. A study was performed to compare B cenocepacia isolates recovered from CF patients receiving care in several European countries and strains isolated from other clinical samples and the environment, with reference isolates from the epidemic B cenocepacia strain PHDC which has so far only been recovered from CF patients and soil in the USA. Methods: A large collection of B cenocepacia isolates, including a large number recovered from CF patients receiving care in several European countries, Canada and the USA, were genotyped by means of randomly amplified polymorphic DNA typing (RAPD) and rep-PCR using the BOX-A1R primer (BOX-PCR). Results: Nineteen Burkholderia cenocepacia isolates cultured from clinical samples in Europe (18 recently recovered from CF patients in France and Italy and one recovered in 1964 from urine in the UK) showed RAPD fingerprinting patterns that were similar to patterns obtained from isolates of B cenocepacia strain PHDC. Subsequent analysis of these isolates using BOX-PCR confirmed that the European isolates and strain PHDC represent the same clone. Conclusion: Strain PHDC represents a second transatlantic B cenocepacia clone capable of colonising patients with CF.
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页码:952 / 954
页数:3
相关论文
共 15 条
[1]   Endemicity and inter-city spread of Burkholderia cepacia genomovar III in cystic fibrosis [J].
Chen, JS ;
Witzmann, KA ;
Spilker, T ;
Fink, RJ ;
LiPuma, JJ .
JOURNAL OF PEDIATRICS, 2001, 139 (05) :643-649
[2]   Diversity and significance of Burkholderia species occupying diverse ecological niches [J].
Coenye, T ;
Vandamme, P .
ENVIRONMENTAL MICROBIOLOGY, 2003, 5 (09) :719-729
[3]   Population structure analysis of Burkholderia cepacia genomovar III:: varying degrees of genetic recombination characterize major clonal complexes [J].
Coenye, T ;
LiPuma, JJ .
MICROBIOLOGY-SGM, 2003, 149 :77-88
[4]   Comparative assessment of genotyping methods for epidemiologic study of Burkholderia cepacia genomovar III [J].
Coenye, T ;
Spilker, T ;
Martin, A ;
LiPuma, JJ .
JOURNAL OF CLINICAL MICROBIOLOGY, 2002, 40 (09) :3300-3307
[5]   Taxonomy and identification of the Burkholderia cepacia complex [J].
Coenye, T ;
Vandamme, P ;
Govan, JRW ;
Lipuma, JJ .
JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (10) :3427-3436
[6]   Molecular epidemiology of Burkholderia species [J].
Coenye, T ;
LiPuma, JJ .
FRONTIERS IN BIOSCIENCE-LANDMARK, 2003, 8 :E55-E67
[7]   EVIDENCE FOR TRANSMISSION OF PSEUDOMONAS-CEPACIA BY SOCIAL CONTACT IN CYSTIC-FIBROSIS [J].
GOVAN, JRW ;
BROWN, PH ;
MADDISON, J ;
DOHERTY, CJ ;
NELSON, JW ;
DODD, M ;
GREENING, AP ;
WEBB, AK .
LANCET, 1993, 342 (8862) :15-19
[8]   Microbial pathogenesis in cystic fibrosis: Mucoid Pseudomonas aeruginosa and Burkholderia cepacia [J].
Govan, JRW ;
Deretic, V .
MICROBIOLOGICAL REVIEWS, 1996, 60 (03) :539-+
[9]   LINKAGE ANALYSIS OF GEOGRAPHIC AND CLINICAL CLUSTERS IN PSEUDOMONAS-CEPACIA INFECTIONS BY MULTILOCUS ENZYME ELECTROPHORESIS AND RIBOTYPING [J].
JOHNSON, WM ;
TYLER, SD ;
ROZEE, KR .
JOURNAL OF CLINICAL MICROBIOLOGY, 1994, 32 (04) :924-930
[10]   An epidemic Burkholderia cepacia complex strain identified in soil [J].
LiPuma, JJ ;
Spilker, T ;
Coenye, T ;
Gonzalez, CF .
LANCET, 2002, 359 (9322) :2002-2003