Two alternative mechanisms of cell separation in staphylococci: One lytic and one mechanical

被引:9
作者
Giesbrecht, P
Kersten, T
Maidhof, H
Wecke, J
机构
[1] Robert Koch-Institut, D-13353 Berlin
关键词
Staphylococcus aureus; cell wall; morphogenesis; cell separation; murosomes; penicillin-splitting system; Wall autolysins;
D O I
10.1007/s002030050439
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Electron microscopy studies revealed two different mechanisms of cell separation in Staphylococcus aureus. Both mechanisms were initiated by the centrifugal lyric action (directed outward from the center) of murosomes, which perforated the peripheral cell wall. Thereafter, during the first type of cell separation, murosomes also lysed large parts of the cross wall proper in the opposite, i.e., centripetal direction, forming spokelike lytic lesions (''separation scars'') next to the most prominent structure of the cross wall, the splitting system. This bidirectional lyric action of murosomes revealed that the staphylococcal cross wall is composed of permanent and transitory parts; transitory parts constituted about one-third of the volume of the total cross wall and seemed to be digested during cell separation. The second mechanism of cell separation was encountered within the splitting system, which has been regarded as the main control unit for lyric cell separation for more than 25 years. The splitting system, however, represents mainly a mechanical aid for cell separation and becomes effective when cell-wall autolytic activities are insufficient.
引用
收藏
页码:239 / 250
页数:12
相关论文
共 23 条
[1]   REGULAR ARRANGEMENT OF WALL POLYMERS IN STAPHYLOCOCCI [J].
AMAKO, K ;
UMEDA, A .
JOURNAL OF GENERAL MICROBIOLOGY, 1979, 113 (AUG) :421-424
[2]  
AMAKO K, 1978, J ELECTRON MICROSC, V27, P147
[3]   SELECTIVE-INHIBITION OF PENICILLIN-BINDING PROTEINS AND ITS EFFECTS ON GROWTH AND ARCHITECTURE OF STAPHYLOCOCCUS-AUREUS [J].
BEISE, F ;
LABISCHINSKI, H ;
GIESBRECHT, P .
FEMS MICROBIOLOGY LETTERS, 1988, 55 (02) :195-201
[4]   ZERO ORDER KINETICS OF CELL-WALL TURNOVER IN STAPHYLOCOCCUS-AUREUS [J].
BLUMEL, P ;
UECKER, W ;
GIESBRECHT, P .
ARCHIVES OF MICROBIOLOGY, 1979, 121 (02) :103-110
[5]   MORPHOGENESIS OF CELL-WALL OF STAPHYLOCOCCI [J].
GIESBRECHT, P ;
WECKE, J ;
REINICKE, B .
INTERNATIONAL REVIEW OF CYTOLOGY-A SURVEY OF CELL BIOLOGY, 1976, 44 :225-318
[6]   A SPECIAL MORPHOGENETIC WALL DEFECT AND THE SUBSEQUENT ACTIVITY OF MUROSOMES AS THE VERY REASON FOR PENICILLIN-INDUCED BACTERIOLYSIS IN STAPHYLOCOCCI [J].
GIESBRECHT, P ;
LABISCHINSKI, H ;
WECKE, J .
ARCHIVES OF MICROBIOLOGY, 1985, 141 (04) :315-324
[7]  
GIESBRECHT P, 1993, FEMS SYMP, P393
[8]  
GIESBRECHT P, 1971, Cytobiologie, V4, P349
[9]   FAN-SHAPED EJECTIONS OF REGULARLY ARRANGED MUROSOMES INVOLVED IN PENICILLIN-INDUCED DEATH OF STAPHYLOCOCCI [J].
GIESBRECHT, P ;
KERSTEN, T ;
WECKE, J .
JOURNAL OF BACTERIOLOGY, 1992, 174 (07) :2241-2252
[10]   A NOVEL, HIDDEN PENICILLIN-INDUCED DEATH OF STAPHYLOCOCCI AT HIGH DRUG CONCENTRATION, OCCURRING EARLIER THAN MUROSOME-MEDIATED KILLING PROCESSES [J].
GIESBRECHT, P ;
KERSTEN, T ;
MAIDHOF, H ;
KRUGER, D ;
BLUMEL, P ;
GROB, H ;
WECKE, J .
ARCHIVES OF MICROBIOLOGY, 1994, 161 (05) :370-383