Tissue distribution of human aldehyde dehydrogenase E3 (ALDH9): Comparison of enzyme activity with E3 protein and mRNA distribution

被引:23
作者
Izaguirre, G
Kikonyogo, A
Pietruszko, R
机构
[1] RUTGERS STATE UNIV,CTR ALCOHOL STUDIES,PISCATAWAY,NJ 08855
[2] RUTGERS STATE UNIV,DEPT BIOCHEM & MOL BIOL,PISCATAWAY,NJ 08855
来源
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 1997年 / 118卷 / 01期
关键词
ALDH9; gamma-aminobutyraldehyde; betaine; GABA; human aldehyde dehydrogenase E3; Northern blot; osmoregulation; tissue distribution;
D O I
10.1016/S0305-0491(97)00022-9
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The tissue distribution of the E3 isozyme of human aldehyde dehydrogenase has been investigated by three methods: enzyme activity assay employing betaine aldehyde as substrate, Western blotting employing E3 isozyme-specific antibodies, and Northern blotting using a human liver E3 cDNA as probe. All three methods showed that E3 isozyme was universally distributed among all tissues tested. The highest levels of the E3 isozyme activity were found in liver, adrenal gland, and kidney. These same tissues also showed highest levels of the E3 protein via the Western blot. This distribution is consistent with the possible physiological role of E3 isozyme in the synthesis of the osmolyte, betaine, and the neurotransmitter, GABA. Northern blot analysis, however, differed from that of enzyme assay and the Western blot in that it showed highest mRNA levels in skeletal and heart muscles, which had low enzyme activities and E3 protein levels. (C) 1997 Elsevier Science Inc.
引用
收藏
页码:59 / 64
页数:6
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