Retrospective identification of hybridogenic walnut plants by SSR fingerprinting and parentage analysis

被引:52
作者
Pollegioni, Paola [1 ]
Woeste, Keith [2 ]
Mugnozza, Giuseppe Scarascia [3 ]
Malvolti, Maria Emilia [1 ]
机构
[1] CNR, Inst Agroenvironm & Forest Biol, I-05010 Porano, Terni, Italy
[2] Purdue Univ, US Forest Serv, USDA, Dept Forestry & Nat Resources,Hardwood Tree Impro, W Lafayette, IN 47907 USA
[3] CRA Dept Agron Forestry & Land Use, I-00184 Rome, Italy
关键词
Juglans nigra; Juglans regia; Hybrids; Microsatellites; Fingerprinting; Parentage analysis; JUGLANS-REGIA L; PATERNITY ANALYSIS; POLLEN DISPERSAL; GENE FLOW; MARKERS; MICROSATELLITES; GERMINATION; POPULATION; PATTERNS; GROWTH;
D O I
10.1007/s11032-009-9294-7
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Juglans x intermedia (Juglans nigra x Juglans regia) is considered the prototype walnut for quality wood production in Europe. Hybridization between the parental species is rare under natural conditions and difficult using controlled pollination because of phenological and genetic incompatibilities. The identification of hybridogenic parents is the first step toward obtaining hybrid progeny. We report the application of microsatellite markers for DNA fingerprinting and parentage analysis of half-sib families collected in a natural mixed population for which no phenological and morphological data were available. Ten nuclear, neutral, simple sequence repeat markers were used to analyse 600 samples. The high levels of polymorphism detected positively influenced the exclusion and identity probabilities. The assignment analysis revealed the presence of 198 diploid J. x intermedia hybrids among the seedling progeny. Maternity checks were performed on all individuals and few errors of sampling were found. Four distinct hybridogenic mother trees were identified, each showing different reproductive success rates. The 198 diploid hybrids belonged to four open-pollinated families based on an analysis of paternity using a likelihood approach. Differential male reproductive success was observed among pollen donors within the research site. Forty-nine of the 198 diploid hybrids detected in four progenies were sired by only three J. regia genotypes. Backward selection might be used to establish new seed orchards for inter-specific F-1 hybrid production using genotypes with demonstrated compatibility.
引用
收藏
页码:321 / 335
页数:15
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